Evaluation of diagnostic pigments to estimate phytoplankton size classes

Limnology and Oceanography: Methods - Tập 18 Số 10 - Trang 570-584 - 2020
Alison Chase1, Sasha J. Kramer2, Nils Haëntjens1, Emmanuel Boss1, Lee Karp‐Boss1, Mimi Edmondson1, Jason R. Graff3
1School of Marine Sciences, University of Maine, Orono Maine, USA
2Interdepartmental Graduate Program in Marine Science, University of California Santa Barbara, Santa Barbara, California, USA
3Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, USA

Tóm tắt

AbstractPhytoplankton accessory pigments are commonly used to estimate phytoplankton size classes, particularly during development and validation of biogeochemical models and satellite ocean color‐based algorithms. The diagnostic pigment analysis (DPA) is based on bulk measurements of pigment concentrations and relies on assumptions regarding the presence of specific pigments in different phytoplankton taxonomic groups. Three size classes are defined by the DPA: picoplankton, nanoplankton, and microplankton. Until now, the DPA has not been evaluated against an independent approach that provides phytoplankton size calculated on a per‐cell basis. Automated quantitative cell imagery of microplankton and some nanoplankton, used in combination with conventional flow cytometry for enumeration of picoplankton and nanoplankton, provide a novel opportunity to perform an independent evaluation of the DPA. Here, we use a data set from the North Atlantic Ocean that encompasses all seasons and a wide range of chlorophyll concentrations (0.18–5.14 mg m−3). Results show that the DPA overestimates microplankton and picoplankton when compared to cytometry data, and subsequently underestimates the contribution of nanoplankton to total biomass. In contrast to the assumption made by the DPA that the microplankton size class is largely made up of diatoms and dinoflagellates, imaging‐in‐flow cytometry shows significant presence of diatoms and dinoflagellates in the nanoplankton size class. Additionally, chlorophyll bis commonly attributed solely to picoplankton by the DPA, but Chl b‐containing phytoplankton are observed with imaging in both nanoplankton and microplankton size classes. We suggest revisions to the DPA equations and application of uncertainties when calculating size classes from diagnostic pigments.

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Tài liệu tham khảo

10.1038/nature08057

10.1038/nclimate2838

10.3389/fmars.2019.00122

10.1016/j.mio.2013.11.002

10.1016/j.rse.2010.09.004

10.1016/j.rse.2015.07.004

10.1016/j.ecolmodel.2010.02.014

10.1002/2014JC009859

10.3389/fmars.2017.00104

10.1016/j.rse.2013.02.013

10.1007/978-1-4899-0762-2_12

10.4319/lo.1994.39.5.1206

10.3389/fmars.2019.00384

10.1016/j.rse.2011.04.025

10.3389/fmars.2017.00126

10.1016/S0967-0645(00)00166-1

10.3389/fmars.2020.00024

10.1016/S0967-0637(99)00004-7

10.1016/j.rse.2019.111387

10.1016/j.dsr.2015.04.006

10.3389/fmars.2018.00209

10.1046/j.1529-8817.1999.3520368.x

10.1016/j.rse.2008.03.011

10.5194/bg-8-311-2011

Hooker S. B. and others.2009. The third SeaWiFS HPLC analysis round‐robin experiment SeaHARRE‐3. NASA/TM‐2009–215849. December 2009.

Jeffrey V. M., 1997, Phytoplankton pigments in oceanography

10.1017/CBO9780511732263.004

10.1002/lno.11259

10.1029/2009JC005303

10.5194/bg-7-3239-2010

10.1029/2019JC015604

10.3389/fmars.2018.00370

Le Quere C., 2005, Ecosystem dynamics based on plankton functional types for global ocean biogeochemistry models, Glob. Chang. Biol., 11, 2016, 10.1111/j.1365-2486.2005.1004.x

10.1038/s41467-018-03376-9

10.1002/jgrc.20137

10.1146/annurev.ecolsys.39.110707.173549

10.1007/s10750-010-0341-5

10.3389/fmars.2019.00196

10.1016/j.rse.2015.05.002

10.3354/meps144265

10.4319/lo.2000.45.3.0569

10.4319/lom.2012.10.278

10.4319/lo.2000.45.1.0098

10.3389/fmars.2017.00041

10.1016/S0272-7714(03)00075-1

10.4319/lom.2007.5.195

10.1364/AO.52.002257

Picheral M. S.Colin andJ.‐O.Irisson.2017. EcoTaxa a tool for the taxonomic classification of images. Available fromhttps://ecotaxa.obs-vlfr.fr. Accessed November 12 2019

10.1016/j.rse.2013.08.004

10.3354/meps07998

10.4319/lo.1972.17.3.0327

10.4319/lo.1978.23.6.1256

10.3354/meps09486

Sommer U., 2017, Benefits, costs and taxonomic distribution of marine phytoplankton body size, J. Plankton Res., 39, 494

10.3390/rs61010089

10.4319/lom.2007.5.204

10.1016/B978-012693018-4/50007-0

10.1029/2005JC003207

10.4319/lo.2008.53.2.0614

10.1016/j.dsr.2008.11.006

10.1016/S0378-4347(00)00603-4

10.1029/1999JC000308

10.1016/j.dsr.2018.04.001

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Limnology and Oceanography: Methods

Tập 18 Số 10

570-584

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