Ethyl pyruvate protects against lipopolysaccharide‐induced white matter injury in the developing rat brain

International Journal of Developmental Neuroscience - Tập 31 - Trang 181-188 - 2013
Yingyan Wang1, Ping Yin1, Shanying Huang2, Jiwen Wang1, Ruopeng Sun1
1Pediatric Department of Qilu Hospital, Shandong University, Jinan, Shandong Province, China
2The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Shandong University Qilu Hospital, Jinan, Shandong Province, China

Tóm tắt

Abstract

The neuroprotective effects of ethyl pyruvate (EP) have been proved in several brain injury models, yet very little is known about its action on neonatal white matter injury. To investigate the effect of EP on white matte damage, a stereotactic intracerebral injection of lipopolysaccharide (LPS, 1 mg/kg) was performed on postnatal day 5 Sprague–Dawley rat pups, and EP was administrated intraperitoneally at a dose of 40 mg/kg immediately, 1 h and 12 h after LPS exposure. Significantly, treatment with EP reduced LPS‐induced ventricle dilation, loss of O4+ and O1+ oligodendrocytes, apoptosis of oligodendrocytes, and hypomyelination. The protective effect of EP was associated with suppressed inflammatory responses, indicated by the inhibition of activation of microglia and astrocytes, as well as the decreased expression of tumor necrosis factor‐alpha (TNF‐α) and interleukin‐1beta (IL‐1β) in rat brains. Also, EP prevented the elevation of cleaved caspase‐3 in periventricular white matter tissue after LPS insult. Taken together, these results suggest that EP confers potent protection against LPS‐induced white matter injury via its anti‐inflammatory and anti‐apoptotic properties.


Tài liệu tham khảo

10.1523/JNEUROSCI.21-04-01302.2001 10.1002/mrdd.20107 10.1161/01.STR.0000254729.27386.05 10.1111/j.1750-3639.2007.00107.x 10.1038/nrn2038 10.1007/s00109-009-0441-8 10.1016/S0006-8993(03)02545-9 10.1203/01.PDR.0000134249.92944.14 10.1016/j.neuroscience.2005.09.023 10.1002/jnr.21052 10.1016/j.bbrc.2010.03.105 10.1097/00008480-200004000-00002 10.1001/archneur.65.10.1291 10.1016/j.neuroscience.2005.02.016 10.1002/dneu.20591 10.1016/j.it.2007.01.005 10.2350/06-01-0024.1 10.1097/SHK.0b013e31818d4073 10.1016/j.cyto.2008.04.007 10.1074/jbc.M202931200 10.1124/jpet.104.079707 10.1212/01.wnl.0000224754.63593.c4 10.1167/iovs.10-7047 10.1016/j.bcp.2010.03.007 10.1016/j.pneurobio.2009.01.003 10.1136/adc.2006.108837 10.1016/j.freeradbiomed.2008.06.009 10.1523/JNEUROSCI.22-07-02478.2002 10.1016/0887-8994(96)00157-9 10.1523/JNEUROSCI.3995-07.2008 10.1189/jlb.0908578 10.1016/j.jsgi.2006.02.011 10.4049/jimmunol.173.6.3916 10.1016/S0165-3806(02)00606-5 10.1016/j.neuroscience.2009.12.040 10.1203/01.pdr.0000199905.08848.55 10.1097/01.shk.0000092697.10326.8b 10.1097/00005072-199501000-00001 10.1002/ana.21359 10.1016/j.nbd.2009.12.010 10.1515/JPM.2007.058 10.1124/jpet.103.056622 Su X., 2011, Beneficial effects of ethyl pyruvate through inhibiting high‐mobility group box 1 expression and TLR4/NF‐kappaB pathway after traumatic brain injury in the rat, Mediators of Inflammation, 2011 10.1097/01.TP.0000151681.07474.2E 10.1073/pnas.192222999 Zoelen M.A., 2007, Intrapulmonary delivery of ethyl pyruvate attenuates lipopolysaccharide‐ and lipoteichoic acid‐induced lung inflammation in vivo, Shock, 28, 570, 10.1097/shk.0b013e31804d40be 10.1016/j.ejphar.2004.12.020 10.1097/ALN.0b013e3181a160d6 10.1016/j.siny.2006.04.002 10.3748/wjg.14.4546 Zhang Y., 2009, Effect of ethyl pyruvate on physical and immunological barriers of the small intestine in a rat model of sepsis, The Journal of Trauma, 66, 1355
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Thông tin xuất bản

International Journal of Developmental Neuroscience

Tập 31

181-188

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