Establishing and maintaining a low-cost zebrafish breeding and behavioral research facility
Tóm tắt
The purpose of this paper is to describe how to establish and maintain a low-cost zebrafish facility that can be used for both teaching and research purposes. The cost of the facility ranges from about $250 to $1,000, depending on the size of the facility and the experiments that will be conducted. The facility plan presented here also has the advantage that it can be disassembled so that it need not function year-round. A detailed list of supplies and materials is provided, along with detailed instructions on how to set up and maintain the adult and breeding portions of the facility. Finally, there is discussion of some of the general uses of a zebrafish facility, including a variety of procedures that are currently being used in behavioral research with this species.
Tài liệu tham khảo
Agbayani, N. (1995). Beam sizes for aquarium stands.Freshwater & Marine Aquarium,18, 51–54.
American Psychological Association (1985).Guidelines for ethical conduct in the care and use of animals [Brochure]. Washington, DC: American Psychological Association.
Barinaga, M. (1990). Zebrafish swimming into the development mainstream.Science,250, 34–35.
Bilotta, J.,Dobis, W. T.,Googe, M. D.,Nunley, W. R., &De-Lorenzo, A. S. (1996). The effects of abnormal lighting conditions on zebrafish visual development.Investigative Ophthalmology & Visual Science,37 (Suppl.), S631.
Bilotta, J., &Powers, M. K. (1992). Weak stimulus generalization using sinusoidal gratings: A cautionary note in animal psychophysics.Behavior Research Methods, Instruments, & Computers,24, 507–510.
Brothers, A. I. (1996).An action spectrum of the zebrafish (Brachydanio rerio). Unpublished manuscript, Temple University, Philadelphia.
Clark, D. T. (1981).Visual responses in developing zebrafish (Brachydanio rerio). Unpublished doctoral dissertation, University of Oregon.
Close, B., Banister, K., Baumans, V., Bernoth, E., Bromage, N., Bunyan, J., Erhardt, W., Flecknell, P., Gregory, N., Hackbarth, H., Morton, D., &Warwick, C. (1997). Recommendations for euthanasia of experimental animals: Part 2.Laboratory Animals,31, 1–32.
Dill, L. M. (1974). The escape response of the zebra danio (Brachydanio rerio): I. The stimulus for escape.Animal Behavior,22, 711–722.
Dobis, W. T., &Googe, M. D. (1996, April).Effects of pH on the development of the zebrafish. Paper presented at the Sigma Xi Student Research Conference, Bowling Green, KY.
Fetsko, L. A., Cohen, E., Sheffield, J. B., &Mote, M. I. (1996). Using associative learning to test zebrafish color vision.Society for Neuroscience Abstracts,22, 886.
Gleason, P. E., Weber, P. G., &Weber, S. P. (1977). Effect of group size on avoidance learning in zebra fish,Brachydanio rerio (Pisces: Cyprinidae).Animal Learning & Behavior,5, 213–216.
Keith-Spiegel, P., Tabachnick, B. G., &Spiegel, G. B. (1994). When demand exceeds supply: Second-order criteria used by graduate school selection committees.Teaching of Psychology,21, 79–81.
Laale, H. W. (1977). The biology and use of zebrafish,Brachydanio rerio in fisheries research.Journal of Fish Biology,10, 121–173.
McCann, L. I., Koehn, D. J., &Kline, N. J. (1971). The effects of body size and body markings on nonpolarized schooling behavior of zebra fish (Brachydanio rerio).Journal of Psychology,79, 71–75.
Mills, D. (1993).You and your aquarium. New York: Knopf.
Moore, C., Hardesty, H. R., Saszik, S., &Bilotta, J. (1997).Effect of physical space on zebrafish development. Unpublished manuscript, Western Kentucky University, Bowling Green.
National Research Council (1996).Guide for the care and use of laboratory animals. Washington, DC: National Academy Press.
Northmore, D. P. M., Volkmann, F. C., &Yager, D. (1978). Vision in fishes: Colour and pattern. In D. J. Mostofsky (Ed.),The behavior of fishes and other aquatic animals (pp. 79–136). New York: Academic Press.
Nunley, W. R., &Bilotta, J. (1996, April).Development of a new apparatus and procedure to measure contrast sensitivity in zebrafish. Paper presented at the annual meeting of the Kentucky Academy of Science, Frankfort, KY.
Ore, S. G., McElroy, D. M., &Hoyt, R. D. (1997). Acute low pH impairs chemoreception and feeding performance in larval fathead minnows (Pimephales promelas).Marine & Freshwater Behavior & Physiology,30, 1–17.
Plous, S. (1996). Attitudes toward the use of animals in psychological research and education: Results from a national survey of psychology majors.Psychological Science,7, 352–358.
Schirone, R. C., &Gross, L. (1968). Effect of temperature on early embryological development of the zebra fish,Brachydanio rerio.Journal of Experimental Zoology,169, 43–52.
Westerfield, M. (1994).The zebrafish book (2nd ed.). Eugene: University of Oregon Press.