Epithelial vertex models with active biochemical regulation of contractility can explain organized collective cell motility

APL Bioengineering - Tập 2 Số 3 - 2018
Sarita Koride1, Andrew J. Loza2, Sean X. Sun1,3,4
1Chemical and Biomolecular Engineering, Johns Hopkins University 1 , Baltimore, Maryland 21218, USA
2Department of Cell Biology, Washington University School of Medicine 2 , St. Louis, Missouri 63110, USA
3Institute of NanoBioTechnology, Johns Hopkins University 4 , Baltimore, Maryland 21218, USA
4Mechanical Engineering, Johns Hopkins University 3 , Baltimore, Maryland 21218, USA

Tóm tắt

Collective motions of groups of cells are observed in many biological settings such as embryo development, tissue formation, and cancer metastasis. To effectively model collective cell movement, it is important to incorporate cell specific features such as cell size, cell shape, and cell mechanics, as well as active behavior of cells such as protrusion and force generation, contractile forces, and active biochemical signaling mechanisms that regulate cell behavior. In this paper, we develop a comprehensive model of collective cell migration in confluent epithelia based on the vertex modeling approach. We develop a method to compute cell-cell viscous friction based on the vertex model and incorporate RhoGTPase regulation of cortical myosin contraction. Global features of collective cell migration are examined by computing the spatial velocity correlation function. As active cell force parameters are varied, we found rich dynamical behavior. Furthermore, we find that cells exhibit nonlinear phenomena such as contractile waves and vortex formation. Together our work highlights the importance of active behavior of cells in generating collective cell movement. The vertex modeling approach is an efficient and versatile approach to rigorously examine cell motion in the epithelium.

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APL Bioengineering

Tập 2 Số 3

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