Enzymatic Determination of Free Carnitine in Milk and Infant Formulas

Journal of AOAC International - Tập 78 Số 1 - Trang 69-74 - 1995
Harvey E. Indyk1, David C Woollard2
1Anchor Products, PO Box 7, Waitoa, New Zealand
2Lynfield Food Services Centre, Ministry of Agriculture and Fisheries, PO Box 41, Auckland, New Zealand

Tóm tắt

Abstract A simple spectrophotometric assay for free carnitine in milk and supplemented infant formulas has been developed. After acid extraction, carnitine is measured enzymatically through its reaction with carnitine acetyltransferase coupled with acetyl coenzyme A and dithiobenzoate (DTNB). The manually performed method is rapid, accurate, and convenient for routine quality-control analysis of infant formulas. The chemistry is also suitable for automation, for greatly enhanced throughput.

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