Enhanced production of recombinant protein inEscherichia coli using silkworm hemolymph

Hockney, J. (1993) Expression systems: A user's guide.Bio/Technology 11: 887–893.

Marino, M. H. (1989) Expression systems for heterologous protein production.BioPharm. 2: 18–33.

Yoon, S. K., W. K. Kang, and T. H. Park (1994) Fed-batch operation of recombinantE. coli containingtrp promoter with controlled specific growth rate.Biotechnol. Bioeng. 43: 995–999.

Yoon, S. K., S. K. Kwon, M. G. Park, W. K. Kang, and T. H. Park (1994) Optimization of recombinantEscherichia coli fed-batch fermentation for bovine somatotropin.Biotechnol. Lett. 16: 1119–1124.

Yoon, S. K., W. K. Kang, and T. H. Park (1996) Regulation oftrp promoter for production of bovine somatotropin in recombinantEscherichia coli fed-batch fermentation.J. ferment. Bioeng. 81: 153–157.

Park, S. and T. H. Park (2000) Analysis of two-stage continuous operation ofEscherichia coli containing bacteriophage γ vector.Bioprocess Eng. 23: 557–563.

Ramisetti, S., H. A. Kang, S. K. Rhee, and C. H. Kim (2003) Production of recombinant hirudin in galactokinase-deficient saccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding.Biotechnol. Bioprocess Eng. 8: 183–186.

Choi, J. I. and S. Y. Lee (2004) High level production of supra molecular weight poly (3-hydroxybutyrate) by metabolically engineeredEscherichia coli.Biotechnol. Bioprocess Eng. 9: 196–200.

Lin, C. S., B. Y. Chen, T. H. Park, and H. C. Lim (1998) Characterization of bacteriophage γ Q mutant for stable and efficient production of recombinant protein inEscherichia coli system.Biotechnol. Bioeng. 57: 529–535.

Kim, T. S. and T. H. Park (2000) Optimization of bacteriophage γ Q-containing recombinantEscherichia coli fermentation process.Bioprocess Eng. 23: 187–190.

Yun, E. S. and T. H. Park (2000) Quantitative measurement of general odorant using electoantennogram of male silkworm moth,Bombyx mori.Biotechnol. Bioprocess Eng. 5: 150–152.

Ha, S. H., T. H. Park, and S. E. Kim (1996) Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture.Biotech. Tech. 10: 401–406.

Ha, S. H. and T. H. Park (1997) Efficient production of recombinant protein inSpodoptera frugiperda/AcNPV system utilizing silkworm hemolymph.Biotechnol. Lett. 19: 1087–1091.

Woo, S. D., W. J. Kim, H. S. Kim, J. Y. Choi, B. R. Jin, and S. K. Kang (1997) Effect of silkworm hemolymph on the expression ofE. coli β-galactosidase in insect cell lines infected with recombinant baculovirus.Mol. Cell 7: 572–574.

Rhee, W. J., E. J. Kim, and T. H. Park (1999) Kinetic effect of silkworm hemolymph on the delayed host cell death in an insect cell-baculovirus system.Biotechnol. Prog. 15: 1028–1032.

Rhee, W. J. and T. H. Park (2000) Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.Biochem. Biophys. Res. Commun. 271: 186–190.

Rhee, W. J. and T. H. Park (2001) Flow cytometric analysis of the effect of silkworm hemolymph on the baculovirus-induced insect cell apoptosis.J. Microbiol. Biotechnol. 11: 853–857.

Rhee, W. J., E. J. Kim, and T. H. Park (2002) Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells.Biochem. Biophys. Res. Commun. 295: 779–783.

Choi, S. S., W. J. Rhee, and T. H. Park (2002) Inhibition of human cell apoptosis by silkworm hemolymph.Biotechnol. Prog. 18: 874–878.

Kim, E. J. and T. H. Park (2003) Anti-apoptosis engineering.Biotechnol. Bioprocess Eng. 8: 76–82.

Kim, E. J., W. J. Rhee, and T. H. Park (2001) Isolation and characterization of an apoptosis-inhibiting component from the hemolymph ofBombyx mori, Biochem. Biophys. Res. Commun. 285: 224–228.

J. H. Miller (1972)Purification of β-Galactosidase.In:Experiments in Molecular Genetics. pp. 398–404. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. USA.

U. K. Laemmli (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4.Nature 227: 680–685.