Enhanced deposition of nitrate alters microbial cycling of N in a subtropical forest soil
Tóm tắt
In China, atmospheric deposition of NO3
− is increasing rapidly. However, information on how microbial N cycling in forest soils may respond to increasing deposition of NO3
− is currently lacking. Determination of process- and pool-specific N transformation rates can provide additional insights into the controls on the production and consumption of inorganic N, and microbial function. Here, we present results from a laboratory 15N tracing study with a soil (0–10 cm) from a subtropical forest receiving fertilization for more than 2.5 years at a rate of 0, 40, and 120 kg NO3
−–N ha−1 year−1. The process- and pool-specific N transformation rates were quantified with a 15N tracing model. The directions of changes in microbial mineralization of labile and recalcitrant organic N were opposite under increased NO3
− additions. Microbial mineralization of labile organic N first decreased, then increased, while microbial mineralization of recalcitrant organic N showed the opposite in response to increasing NO3
− additions. Ammonium immobilization into labile and recalcitrant organic N was not changed by increased NO3
− additions. Nitrate additions did not affect heterotrophic and gross nitrification, but stimulated autotrophic nitrification. Nitrate immobilization decreased under increased NO3
− additions, with a greater reduction under low NO3
− addition treatment compared to high NO3
− addition treatment. Overall, our results reflect a contrasting change in microbial mineralization of liable and recalcitrant organic N under increased NO3
− additions, and a contrasting effect of low and high NO3
− additions on microbial mineralization of liable and recalcitrant organic N. It also has implications for our understanding of NO3
– deposition-induced nonlinear changes in net production and loss of NO3
− in subtropical/tropical forest soils.
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