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Sự bao bọc của axit docosahexaenoic (DHA) bằng cách sử dụng các protein có nguồn gốc thực phẩm tự lắp ráp cho các chức năng sinh học hiệu quả
Tóm tắt
Axit docosahexaenoic (DHA; 22n-6) có nhiều chức năng sinh học, bao gồm hoạt động chống oxy hóa và cải thiện tình trạng tăng triglycerid máu. Tuy nhiên, việc ứng dụng DHA đã bị hạn chế do độ tan trong nước kém và dễ bị oxy hóa. Ở đây, ovalbumin (O), myosin (M), globulin đậu nành 7S (S), và β-lactoglobulin (β), đã được thủy phân bởi chymotrypsin, tự lắp ráp thành các micelle, tương ứng. Việc thêm DHA vào micelle một cách tăng dần đã gây ra hiện tượng quenching huỳnh quang nội sinh của các micelle O, M, S, và β, ngụ ý việc kết hợp thành công DHA vào lõi kỵ nước của các micelle (O (DHA), M (DHA), S (DHA), và β (DHA)). Kết quả cho thấy các micelle cung cấp sự ổn định không gian và cải thiện độ tan, và ổn định trước nhiệt độ và ánh sáng cực tím (UV) cho DHA. Sự hấp thu DHA từ M (DHA), β (DHA), O (DHA), và S (DHA) lần lượt cao hơn 3,27-, 3,91-, 2,7- và 3,95 lần so với DHA thông qua các tế bào Caco-2. Việc bao bọc trong các micelle đã làm tăng độ tan trong nước và sự hấp thu DHA, điều này nâng cao khả năng phòng vệ chống oxy hóa nội sinh của tế bào. Trong khi đó, sự gia tăng hấp thu DHA cũng đã được xác nhận bởi các tế bào HepG2, và các micelle O, M, S và β đã được chứng minh là làm tăng sự hấp thu DHA để giảm sự lắng đọng lipid. Các phát hiện của chúng tôi mạnh mẽ hỗ trợ khả năng rằng các micelle O, M, S và β có thể được coi là một chất mang để tải DHA.
Từ khóa
#Docosahexaenoic acid #Micelle #Solubility #Stability #Cellular uptakeTài liệu tham khảo
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