Emulsions Based on the Interactions Between Lactoferrin and Chitosans
Tóm tắt
In this work, purification of lactoferrin from whey was performed with high recovery rate. Lactoferrin was then exploited in the preparation of food emulsions. Two tertiary emulsions, formed by olive oil, lecithin, chitosan, and lactoferrin, were compared: both the emulsions showed similar turbidity and stability. In the secondary emulsion formed by oil/lecithin/chitosan, the pH was increased to 9 before addition of lactoferrin. Then, lactoferrin was added, and the pH was stabilized above pH 9. Lactoferrin was found in amounts of 1 to 2.5 mg/ml in the multiple experiments. A fraction of the added lactoferrin was also present in a milky layer above the emulsion layer. This was, to our knowledge, the first study of emulsions made exploiting the interactions between lactoferrin and chitosan. It was noted that chitosan droplets remained soluble, although the hydrocolloid solubility occurs at pH lower than 5.9. These results showed the feasibility of manufacturing lactoferrin-based emulsions as functional foods.
Tài liệu tham khảo
Friberg, K. Larson, J. Sjobolm, Food Emulsions, 4th edn. (Marcel Dekker, New York, 2004)
Guzey, D.J. McClements, Advances Colloid Interface Sci. 128–130, 227–248 (2006)
Rosa, G. Sala, T. van Vliet, F. van de Velde, J. Texture Stud. 37, 516–537 (2006)
Ye, H. Singh, Food Biophys. 2, 125–132 (2007)
Ye, H. Singh, J. Colloid Interface Sci. 295, 249–254 (2006)
Kruzel, A. Bacsi, B. Choudhury, S. Sur, I. Boldogh, Immunology 119, 159–166 (2006)
Haney, F. Lau, H.J. Vogel, Biochim. Biophys. Acta 1768, 355–2364 (2007)
Kruzel, Y. Harari, D. Mailman, J.K. Actor, M. Zimecki, Clin. Exp. Immunol. 130, 25–31 (2002)
Choe, S.W. Lee, J. Cell. Biochem. 76, 30–36 (1999)
Crouch, K.J. Slater, J. Fletcher, Blood 80, 235–240 (1992)
Togawa, H. Nagase, K. Tanaka et al., J. Gastroenterol Hepatol. 17, 1291–1298 (2002)
Zimecki, J. Mazurier, G. Spik, J.A. Kapp, Immunology 86, 122–127 (1995)
Weinberg, Expert Opin. Investig. Drugs 12, 841 – 851 (2003)
Soejima, K. Yamaguchi, T. Yamamoto et al., J. Dairy Sci. 74, 100–105 (2007)
Uzzan, Y. Nechrebeki, T.P. Labuza, J. Food Sci. 72, E109 –E 114 (2007)
K. Plate, S. Beutel, H. Buchholz, W. Demmer et al., J. Chromatogr. A. 1117, 81–86 (2006)
S.F. Ausar, D. Bianco, R.G. Badini, L.F. Castagna et al., J. Dairy Sci. 84, 361–369 (2001)
U.K. Laemmli, Nature 227, 680–685 (1970)
H. Soyeurt, F.G. Colinet, V.M. Arnould et al., J. Dairy Sci. 90, 4443–4450 (2007)
T. Harnsilat, R. Pongsawatmanit, D.J. McClements, J. Agric. Food Chem. 54, 5540–5547 (2006)
E. Dickinson, Food Hydrocolloids 17, 25–39 (2003)
S. Magdassi, U. Bach, K.Y. Mumcuoglu, J. Microencapsul. 14, 189–195 (1997)
D.J. McClements, Langmuir 21, 9777–9785 (2005)