Electroporation: A general phenomenon for manipulating cells and tissues
Tóm tắt
Electroporation is a fascinating cell membrane phenomenon with several existing biological applications and others likely. Although DNA introduction is the most common use, electroporation of isolated cells has also been used for (1) introduction of enzymes, antibodies, and other biochemical reagents for intracellular assays; (2) selective biochemical loading of one size cell in the presence of many smaller cells; (3) introduction of virus and other particles; (4) cell killing under nontoxic conditions; and (5) insertion of membrane macromolecules into the cell membrane. More recently, tissue electroporation has begun to be explored, with potential applications including (1) enhanced cancer tumor chemotherapy, (2) gene therapy, (3) transdermal drug delivery, and (4) noninvasive sampling for biochemical measurement. As presently understood, electroporation is an essentially universal membrane phenomenon that occurs in cell and artificial planar bilayer membranes. For short pulses (
Từ khóa
Tài liệu tham khảo
Chang DC, 1992, “Sowers, Guide to Electroporation and Electrofusion.”
Blank M, 1992, “Proceedings of the First World Congress for Electricity and Magnetism in Biology and Medicine.”
Neumann E, 1992, “Colloid and Molecular Electro‐Optics.”, 197
WeaverJC(1992):Electroporation: A dramatic nonthermal electric field phenomenon. In Proceedings of the First World Congress for Electricity and Magnetism in Biology and Medicine Lake Buena Vista Florida June 14–19.
Chang DC, 1992, “Guide to Electroporation and Electrofusion.”, 9
WeaverJC AstumianRD(1992):Estimates for ELF effects: Noise‐based thresholds and the number of experimental conditions required for empirical searches. Bioelectromagnetics (in press).
Pastushenko VF, 1982, Stabilization of conducting pores in BLM by electric current, Gen Physiol Biophys, 1, 43
PrausnitzMR BoseVG LangerRS WeaverJC(1993):Electroporation of human skin: A novel approach to transdermal drug delivery. (Submitted.)
Sugar IP, 1981, The effects of external fields on the structure of lipid bilayers, J Physiol (Paris), 77, 1035
Weaver JC, 1992, “Guide to Electroporation and Electrofusion.”, 91
Sukharev SI, 1983, Reversible electrical breakdown of cholesterol‐containing bilayer lipid membranes modified with holothurin A, Biophysics, 28, 451
FreemanSA WangMA WeaverJC(1993):Theory of electroporation for a planar bilayer membrane: Predictions of the fractional aqueous area change in capacitance and pore–pore separation. (Submitted.)
Chakrabarti R, 1989, Transfer of monoclonal antibodies into mammalian cells by electroporation, J Biol Chem, 264, 15494, 10.1016/S0021-9258(19)84857-3
PrausnitzMR LauBS MilanoCD ConnerS LangerRS WeaverJC(in preparation):A quantitative study of electroporation showing a plateau in net molecular transport.
MycueD ZahnJ ZahnM WeaverJC(in press):Interaction mechanism of electric fields with cells. In: “Alteration of Binding Site Access of Membrane Proteins ” Proceedings of the First World Congress for Electricity and Magnetism in Biology and Medicine Lake Buena Vista FL June 14–19 1992.
Mir LM, 1992, Electrochemotherapy tumor treatment is improved by interleukin‐2 stimulation of the host's defenses, Eur Cyto Net, 3, 331