Electron microscopy after rapid freezing on a metal surface and substitution fixation

Wiley - Tập 149 Số 3 - Trang 381-385 - 1964
A. Van Harreveld1, Jane Crowell1
1Kerckhoff Laboratories of the Biological Sciences, California Institute of Technology, Pasadena, California

Tóm tắt

AbstractA method for rapid freezing is described in which use is made of the good heat conducting properties of silver. The freezing was accomplished by bringing the tissue in contact with a polished silver surface at the temperature of liquid nitrogen either at atmospheric or reduced pressure. Helium gas flowing over this surface prevented the condensation of water or air on the silver. After freezing the tissue was placed in a substituting solvent. The best results were obtained with 2% osmium tetroxide in acetone at −85°C. The ultrastructure of the tissue was well preserved in a narrow surface layer only.

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Tài liệu tham khảo

10.1083/jcb.12.1.198

10.1083/jcb.8.3.639

Bullivant S.1962Consideration of membranes and associated structures after cryofixation. Proc. 5th Internat. Congress for Electron Microscopy R‐2.

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10.1083/jcb.11.3.729

Rebhun L. I. andH. T.Gagné1962Some aspects of freeze‐substitution in electron microscopy. Proc. 5th Internat. Congress for Electron Microscopy L2.

10.1083/jcb.8.3.575

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Wiley

Tập 149 Số 3

381-385

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