Electrochemical properties of heme-protein in lauric acid films and its application as a biosensor
Tóm tắt
In this research, the enhancement of electron-transfer activity of hemoglobin (Hb) in lauric acid film was investigated for the first time. This type of composite film was made on a glassy carbon electrode by a casting method. Cyclic voltammetric result of the modified electrode displays a well-defined redox peak, which was attributed to the direct electrochemical response of Hb. Our results illustrate that Hb exchange electrons directly with electrode and exhibits the characteristics of peroxidase. When we use this modified electrode as a biosensor, it gives excellent performance in the electrocatalytic reduction of hydrogen peroxide (H2O2). The parameters such as pH and applied potential of the biosensor influencing in H2O2 detection were optimized carefully. Through the optimal conditions, the proposed biosensor shows the linear range for H2O2 determination was from 1×10−5 to 1.25×10−4 mol L−1 with a detection limit of 1×10−7 mol L−1. The biosensor retained more than 90% of the initial response after 14 d.
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