Effects on yield and nutrition of mycorrhizal and nodulated Pueraria phaseoloides exerted by P-solubilizing rhizobacteria
Tóm tắt
We studied the effect of bacteria involved in rock phosphate (four isolates), iron phosphate (two isolates), and aluminium phosphate (two isolates) solubilization, and two phytate-mineralizing bacteria in terms of their interaction with two Glomus spp. on Pueraria phaseoloides growth and nutrition. The plant —Rhizobium sp. — mucorrhiza symbiosis system may increase in yield and nutrition in association with specific rhizosphere bacteria that solubilize calcium, iron, and aluminium phosphates. No benefit from phytate-mineralizing bacteria was found under these experimental conditions. P. phaseloides growth responses were influenced in different ways by specific combinations of the selected bacteria and arbuscular mycorrhizal fungi. Considerable stimulation of nutrient uptake was observed with fungus-bacteria combinations of Azospirillum sp. 1, Bacillus sp. 1 or Enterobacter (spp. 1 or 2) associated with G. mosseae. The fact that Bacillus sp. 1, a calcium-phosphate solubilizing isolate, positively interacted with G. mosseae and negatively with G. fasciculatum is an indication of specific functional compatibility between the biotic components integrated in the system. From our results, the interactions between bacterial groups able to solubilize specific phosphate and mycorrhizal fungi cannot be interpreted as occurring only via P solubilization mechanisms since no generalized effect was obtained. Iron-phosphate solubilizing microorganisms were more active alone than in dual associations with Glomus sp., but the aluminium-phosphate dissolving isolates positively interacted in mycorrhizal plants. Further work is needed in this area in order to elucidate the mechanisms that affect rhizosphere microorganism interactions. G. mosseae was more effective but less infective than G. fasciculatum in most of the combined treatments.
Tài liệu tham khảo
Abbott LK, Robson AD (1981) Infectivity and effectiveness of vesicular-arbuscular mycorrhizal fungi: Effect of inoculum type. Aust J Agric Res 32:631–639
Allen ON (1957) Experiments in soil bacteriology. Burgess N N Pub Co, Minneapolis, MN
Arora D, Gaur AC (1979) Microbial solubilization of different inorganic phosphates. Exp Biol 17:1258–1261
Azcón R (1987) Germination and hyphal growth of Glomus mosseae in vitro: Effects of rhizosphere bacteria and cell-free culture media. Soil Biol Biochem 19:417–419
Azcón R (1989) Selective interaction between free-living rhizosphere bacteria and vesicular-arbuscular mycorrhizaf fungi. Soil Biol Biochem 5:639–644
Azcón R (1993) Growth and nitrition of nodulated mycorrhizal and non-mycorrhizal Hedysarum coronarium as a result of treatment with fractions from a plant growth-promoting rhizobacteria. Soil Biol Biochem 25:1037–1042
Azcón R, Barea JM, Hayman D (1976) Utilization of rock phosphate in alkaline soils by plants inoculated with mycorrhizal fungi and phosphate-solubilizing bacteria. Soil Biol Biochem 8:135–138
Azcón-Aguilar C, Gianinazzi-Pearson V, Fardeau JC, Gianinazzi S (1986) Effect of vesicular-arbuscular mycorrhizal fungi and phosphate-solubilizing bacteria on growth and nutrition of soybean in a neutral-calcareous soil amended with 32P−45Ca-tricalcium phosphate. Plant and Soil 96:3–15
Baas R, Van Dijk C, Troelstra RS (1989) Effects of rhizosphere soil, vesicular-arbuscular mycorrhizal fungi and phosphate on Plantago major L. ssp. pleiosperma Pilger*. Plant and Soil 113: 59–67
Banik S, Dey BK (1982) Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate-solubilizing microorganisms. Plant and Soil 69:353–364
Barber SA (1984) Soil nutrient bioavailability. Wiley and Sons, New York
Barea JM, Zzcón R, Hayman DS (1975) Possible synergistic interactions between Endogone and phosphate-solubilizing bacteria in low phosphate soils. In: Mosse B, Sanders FE, Tinker PB (eds) Endomycorrhiza. Proc Symp Univ Leeds, July 1974, Academic Press, New York
Barea JM, Azcón R, Azcón-Aguilar C (1983) Interactions between phosphate solubilizing bacteria and VA mycorrhiza to improve plant utilization of rock phosphate in non acidic soils. In: 3rd International Congress on Phosphorus Compounds Proceedings, Brussels, Belgium. Institut Mondial du Phosphate, Casablanca, pp 127–144
Bolan NS, Robson AD, Barrow NJ (1987) Effects of vesicular-arbuscular mycorrhiza on the availability of iron phosphates to plants. Plant and Soil 99:401–410
Dalal RC (1977) Soil organic phosphorus. Adv Agron 29:83–117
Datta M, Banik S, Gupta RK (1982) Studies on the efficacy of a phytohormone producing phosphate solubilizing Bacillus firmus in augmenting paddy yield in acid soils of Nagaland. Plant and Soil 69:365–373
Giovanetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection on roots. New Phytol 84:489–500
Hepper C, O'Shea J (1984) Vesicular-arbuscular mycorrhizal infection in lettuce (Lactuca sativa) in relation to calcium supply. Plant and Soil 82:61–68
Kucey RMN, Janzen HH, Leggett ME (1989) Microbially mediated increases in plant-available phosphorus. Adv Agron 42: 199–228
Lachica M, Aguilar A, Yañez J (1973) Analisis foliar.Métodos analíticos utilizados en la Estación Experimental del Zaidín. Anal Edaf Agrobiol 32:1033–1047
Linderman RG (1992) Vesicular-arbuscular mycorrhizae and soil microbial interactions. In: Bethlenfalvay GJ, Linderman RG (eds) Mycorrhizae in sustainable agriculature. Am Soc Agron Spec Publ, Madison, Wis, pp 1–28
Louw HA, Webley DM (1959) A study of soil bacteria dissolving certain mineral phosphate fertilizers and related compounds. J Appl Bacteriol 22:227–233
Paulitz TC, Linderman RG (1989) Interactions between fluorescent pseudomonads and VA mycorrhizal fungi. New Phytol 113: 37–45
Phillips JM, Hayman D (1970) Improved procedures for clearing roots and staining parasitic and VA mycorrhizal fungi and rapid assessment of infection. Trans Br Mycol Soc 55:158–161
Piccini D, Azcón R (1987) Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizal fungi on the utilization of Bayovar rock phosphate by alfalfa plants using a sand-vermiculite medium. Plant and Soil 101:45–50
Rambelli A (1973) The rhizosphere of mycorrhizae. In: Marks GC, Kozlowski TT (eds) Ectomycorrhizae, their ecology and physiology. Academic Press, London New York, pp 299–343
Ramos A, Callao V (1967) El empleo de la solubilización de fosfatos en placa como técnica diferencial bacteriana. Microbiol Españ 20:1–2
Sanders FE, Tinker PB (1971) Mechanism of absorption of phosphate from soil by Endogone mycorrhizas. Nature (London) 233:278–279
Sperber J (1958) Solution of apatite by soil microorganisms producing organic acids. Aust J Agric Res 9:782–787
Stevenson FJ (1986) Cycles of soil carbon, nitrogen, phosphorus, sulphur, micronutrients. Wiley and sons, New York