Effects of neonatal exposure to a glyphosate-based herbicide on female rat reproduction

Reproduction - Tập 152 Số 5 - Trang 403-415 - 2016
Paola I. Ingaramo1, Jorgelina Varayoud1, María M. Milesi1, Marlise Guerrero Schimpf1, Mónica Muñoz‐de‐Toro1, Enrique H. Luque1
1Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral -Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Argentina

Tóm tắt

In this study, we investigated whether neonatal exposure to a glyphosate-based herbicide (GBH) alters the reproductive performance and the molecular mechanisms involved in the decidualization process in adult rats. Newborn female rats received vehicle or 2 mg/kg/day of a GBH on postnatal days (PND) 1, 3, 5 and 7. On PND90, the rats were mated to evaluate (i) the reproductive performance on gestational day (GD) 19 and (ii) the ovarian steroid levels, uterine morphology, endometrial cell proliferation, apoptosis and cell cycle regulators, and endocrine pathways that regulate uterine decidualization (steroid receptors/COUP-TFII/Bmp2/Hoxa10) at the implantation sites (IS) on GD9. The GBH-exposed group showed a significant increase in the number of resorption sites on GD19, associated with an altered decidualization response. In fact, on GD9, the GBH-treated rats showed morphological changes at the IS, associated with a decreased expression of estrogen and progesterone receptors, a downregulation of COUP-TFII (Nr2f2) and Bmp2 mRNA and an increased expression of HOXA10 and the proliferation marker Ki67(Mki67) at the IS. We concluded that alterations in endometrial decidualization might be the mechanism of GBH-induced post-implantation embryo loss.

Từ khóa


Tài liệu tham khảo

10.1016/j.chemosphere.2013.06.041

10.1289/ehp.01109851

10.1002/ps.775

10.1016/j.neuint.2012.09.003

10.1007/s00244-006-0154-8

10.1095/biolreprod.109.078543

10.1051/medsci/20143006014

10.1177/002215540305100503

10.1016/j.etap.2007.08.011

10.1186/1471-2199-8-113

10.1016/j.tiv.2011.12.009

10.1016/j.placenta.2003.11.007

10.1095/biolreprod63.1.188

10.1016/S0378-4274(02)00483-6

10.1007/s00204-006-0170-5

10.1530/REP-08-0539

10.1016/S1642-431X(12)60080-1

10.1016/j.tox.2009.06.006

Greenlee, 2003, Risk factors for female infertility in an agricultural region, Epidemiology, 14, 429, 10.1097/01.EDE.0000071407.15670.aa

10.1093/humrep/

10.1038/nbt0993-1026

2015, Glyphosate, Some Organophosphate Insecticides and Herbicides: Diazinon, Glyphosate, Malathion, Parathion, and Tetrachlorvinphos, 112, 1

10.1016/j.mce.2016.02.011

10.1186/1477-7827-1-44

10.1016/j.reprotox.2012.02.002

10.1177/1933719111413298

10.1371/journal.pgen.0030102

10.1530/REP-13-0654

10.1210/me.2009-0531

10.1128/MCB.00342-07

10.1210/en.2015-2031

10.1074/jbc.M704723200

10.1210/me.13.6.1005

10.1093/molehr/gan023

10.1371/journal.pone.0124756

10.1016/j.mce.2014.11.028

10.1677/JOE-07-0014

10.1016/j.reprotox.2009.06.012

10.1016/j.reprotox.2010.08.004

10.1159/000146639

10.1210/en.140.6.2704

10.1210/me.2015-1142

10.1016/j.reprotox.2011.12.011

10.1016/j.envpol.2008.01.015

10.1095/biolreprod39.5.999

10.1093/molehr/gah147

10.1095/biolreprod.101.002402

10.1016/j.brainresbull.2006.12.004

10.1289/ehp.7728

10.1016/j.reprotox.2011.06.118

10.1530/REP-14-0567

10.1016/j.reprotox.2010.07.008

10.1007/s00204-011-0788-9

10.1007/s00204-009-0494-z

10.1016/j.mce.2011.09.035

10.1016/j.jri.2009.06.262

10.1210/me.2005-0019

10.1210/en.140.11.5310

10.1016/S0925-4773(01)00614-1

10.1016/j.fct.2013.05.057

US EPA 1993 EPA Reregistration eligibility document, Glyphosate, Office of Prevention, Pesticides and Toxic Substances Washington, DC.

10.1210/en.2009-1037

10.1210/en.2008-0651

10.1016/S0074-7696(08)61637-X

10.1016/j.mce.2011.10.028

WHO 1994 Glyphosate. In Environmental Health Criteria, 3rd edn. Geneva, Switzerland: WHO.

10.1080/10937404.2012.632361

10.1210/en.2004-1605

10.1016/j.yfrne.2010.06.003

10.1016/j.febslet.2014.05.045