Effects of nanoimprinted patterns in tissue-culture polystyrene on cell behavior

Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena - Tập 23 Số 6 - Trang 2984-2989 - 2005
Walter Hu1, Evelyn K. F. Yim2, Ronald M. Reano1, Kam W. Leong2, S. W. Pang1
1The University of Michigan Solid State Electronics Laboratory, Department of Electrical Engineering and Computer Science, , Ann Arbor, Michigan 48109
2Johns Hopkins School of Medicine Department of Biomedical Engineering, , Baltimore, Maryland 21205

Tóm tắt

Tissue engineering seeks to develop functional tissues in a biomimetic environment in vitro. As the extracellular environment in vivo is composed of numerous nanostructures, fabrication of nanostructured substrates will be valuable for tissue engineering applications. In this article, we report a simple nanoimprint lithography (NIL) process to pattern nanostructures directly on tissue-culture polystyrene plates. By repeating this NIL process, three-dimensional scaffolds consisting of multiple-layer nanostructures were also fabricated. Bovine pulmonary artery smooth muscle cells were cultured on imprinted gratings ranging from 350nmto10μm. The smooth muscle cells attached and proliferated well on these imprinted substrates without additional surface treatment. Cell elongation and alignment were observed on the micro- and nanopatterns, with the effect significantly more pronounced on the nanostructures.

Từ khóa


Tài liệu tham khảo

2000, Cell Tissue Res., 299, 39, 10.1007/s004410050004

2002, Cells Tissues Organs, 170, 251, 10.1159/000047929

1991, J. Cell. Sci., 99, 73, 10.1242/jcs.99.1.73

1997, Biomaterials, 18, 1573, 10.1016/S0142-9612(97)00144-0

2003, Biomaterials, 24, 3427, 10.1016/S0142-9612(03)00208-4

2004, Biomaterials, 25, 53, 10.1016/S0142-9612(03)00471-X

1999, Biomaterials, 20, 2363, 10.1016/S0142-9612(99)00165-9

2003, Biomaterials, 24, 2533, 10.1016/S0142-9612(03)00052-8

1995, Science, 269, 664, 10.1126/science.7624795

1998, Phys. World, 11, 31

2003, Macromol. Mater. Eng., 288, 76, 10.1002/mame.200290037

2003, J. Vac. Sci. Technol. B, 21, 683, 10.1116/1.1545753

2003, Microelectron. Eng., 67, 162, 10.1016/S0167-9317(03)00067-4

1998, Biomaterials, 19, 1405, 10.1016/S0142-9612(98)00021-0

2002, Tissue Eng., 8, 1089, 10.1089/107632702320934182

1996, Biomaterials, 17, 1417, 10.1016/0142-9612(96)87284-X

2004, J. Vac. Sci. Technol. B, 22, 1711, 10.1116/1.1763897

1996, Science, 272, 85, 10.1126/science.274.5284.85

2002, J. Vac. Sci. Technol. B, 20, 2872, 10.1116/1.1523404

2002, J. Vac. Sci. Technol. B, 20, 2881, 10.1116/1.1526355

2003, J. Vac. Sci. Technol. B, 21, 2742, 10.1116/1.1624258

2004, J. Vac. Sci. Technol. A, 22, 1873, 10.1116/1.1756882

2005, Biomaterials, 26, 4281, 10.1016/j.biomaterials.2004.10.040

2004, Physiol. Rev., 84, 767, 10.1152/physrev.00041.2003

Thông tin
Thông tin xuất bản

Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena

Tập 23 Số 6

2984-2989

Thông tin tác giả