Effects of freeze-thaw cycles on physicochemical properties and structure of cooked crayfish (Procambarus clarkii)

Food Production, Processing and Nutrition - Tập 4 - Trang 1-14 - 2022
Jiping Han1,2,3, Yingjie Sun1,2,3, Rongxue Sun1,3, Tao Zhang4, Cheng Wang1,3, Ning Jiang1,2,3
1Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, People’s Republic of China
2College of Food and Bioengineering, Jiangsu University, Zhenjiang, People’s Republic of China
3Integrated Scientific Research Base for Preservation, Storage and Processing Technology of Aquatic Products of the Ministry of Agriculture and Rural Areas, Nanjing, People’s Republic of China
4College of Food Science and Engineering, Nanjing University of Finance & Economics, Nanjing, People’s Republic of China

Tóm tắt

To explore the damage mechanisms of freeze-thaw cycles on cooked crayfish in frozen storage, changes in the physicochemical properties and structure of cooked crayfish during the freeze-thaw cycles were investigated. The physicochemical properties of cooked crayfish changed significantly after five freeze-thaw cycles. The moisture content, water holding capacity, pH, and textural properties were decreased, while the total color difference, drip loss, and protein and lipid oxidation were significantly increased (P < 0.05). LF-NMR and MRI verified the water loss, and SDS-PAGE showed denaturation/degradation of myofibrillar proteins (MPs). Multiple freeze-thaw cycles promoted the transition from α-helix to β-turn in the secondary structure, the unfolding of tertiary structure, and a significant change in the chemical forces of MPs. SEM results revealed a disruption in the microstructure of muscle fibers. Repeated freeze-thaw cycles reduced the moisture content and distorted the structure of MPs in cooked crayfish, resulting in the disruption of physicochemical properties and its structure.

Tài liệu tham khảo

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