Effects of calcium on protein turnover of incubated muscles from mdx mice

American Journal of Physiology - Endocrinology and Metabolism - Tập 260 Số 4 - Trang E594-E598 - 1991
P A MacLennan1, Anne McArdle1, R. H. T. Edwards1
1Department of Medicine, University of Liverpool, United Kingdom.

Tóm tắt

Mdx mice have a genetic defect similar to that which causes Duchenne muscular dystrophy in humans. The influence of calcium on muscle protein metabolism of mdx and wild type (C57BL/10) mice was examined in vitro. Incubation of mdx muscles in a medium containing calcium at a concentration of 2.0 mM (but not 0.2 mM) resulted in proteolytic rates that were greater than those of C57BL/10 muscles. At 2.0 mM extracellular calcium, mdx muscle proteolysis was attenuated by thiol protease inhibitors but not by the weak base methylamine. Protein synthetic rates were higher in incubated mdx muscles than in incubated C57BL/10 muscles, but no effect of extracellular calcium concentration was observed in either strain. These data suggest that mdx mice have an abnormality of muscle calcium handling, which results in activation of nonlysosomal proteolytic processes but does not exert acute effects on protein synthetic rate.

Từ khóa


Tài liệu tham khảo

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Thông tin
Thông tin xuất bản

American Journal of Physiology - Endocrinology and Metabolism

Tập 260 Số 4

E594-E598

Thông tin tác giả