Effects of an Essential Oil from the Bark of Croton cajucara Benth. on Experimental Gastric Ulcer Models in Rats and Mice

Journal of Pharmacy and Pharmacology - Tập 51 Số 3 - Trang 341-346 - 1999
Clélia Akiko Hiruma-Lima1, J.S. Gracioso2, Domingos Sávio Nunes3, Arm Souza Brito4
1Instituto de Biologia, Fund, Universidade do Tocantins, Porto National, Tocantins, Brasil
2Faculdade de Ciências MÉdicas, Departamento de Farmacologia Universidade Estadual de Campinas , Campinas, São Paulo,
3Departamento de Química, Universidade Estadual de Ponta Grossa , Ponta Grossa, Paraná,
4Departamento de Fisiologia, Instituto de Biologia, Universidade Estadual de Campinas , Campinas, São Paulo,

Tóm tắt

Abstract Croton cajucara Benth. (Euphorbiaceae) is widely used in Amazonian folk medicine for the treatment of a wide range of gastrointestinal symptoms. The essential oil from its bark was investigated for acute toxicity in mice and for its ability to prevent the formation of ulceration of the gastric mucosa in different models of experimentally induced gastric ulcer in mice and rats. When previously administered orally at a dose of 100mg kg-1, the essential oil significantly reduced (P < 0.01) the gastric injury induced by hypothermic restraint stress (48%), indomethacin (47%), ethanol (86%) and pylorus ligature models (87%) in rats. In the HCl/ethanol-induced gastric ulcer model in mice, at oral doses of 100 and 200mg kg-1 the essential oil from C. cajucara significantly reduced (P < 0.01) the formation of gastric lesions by 52% and 67%, respectively, when compared with the control group. In rats submitted to pylorus ligature, the essential oil given orally increased the volume of gastric juice when compared with the control group (P < 0.01). When the essential oil (100mg kg-1) was administered intraduodenally to mice, significant modifications were found in gastric parameters such as pH and total acid content after oil treatment. We observed significant changes (P < 0.01) in gastric juice parameters such as an increase in volume and a decrease in gastric acidity (pH and total acid content). The acute toxicologic effects of the essential oil from C. cajucara were assessed in mice. The LD50 values were 9.3g kg-1 by the oral route and 680mg kg-1 by the intraperitoneal route. The good yield of essential oil obtained from dried C. cajucara bark (1%) as well as its anti-ulcerogenic activity and low toxicity suggest that pharmacological studies of this substance as a potential new anti-ulcerogenic drug are warranted.

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Tài liệu tham khảo

Desai, 1994, Gastric and duodenal antiulcer activity of sulpiride, a dopamine D2 receptor antagonist, in rats, Agents Actions, 42, 149, 10.1007/BF01983482

Di Stasi, 1989, Plantas Medicinais da Amazônia, 127

Droy-Lefaix, 1988, Prostaglandins: biology and chemistry of prostaglandins and related eicosanoids, Prostaglandins: Biology and Chemistry of Prostaglandins and Related Eicosanoids, 345

Goa, 1987, Enprostil: a preliminary review of its pharmacodynamics and pharmacokinetic properties and therapeutic efficacy in the treatment of peptic ulcer disease, Drugs, 3, 539, 10.2165/00003495-198734050-00003

Hayden, 1978, Inhibitors of gastric lesion in the rat, J. Pharm. Pharmacol., 30, 244, 10.1111/j.2042-7158.1978.tb13214.x

Hiruma-Lima, 1998, Antiulcerogenic mechanisms of dehydrocrotonin, a diterpenelactone obtained from Croton cajucara Benth, Planta Med.

Konturek, 1984, Prostaglandins in peptic ulcer disease: effect of non-steroidal anti-inflammatory compounds, Scand. J. Gastroenterol., 92, 250

Koo, 1986, Effect of verapamil, carbenoxolone and N-acetylcysteine on gastric wall mucus and ulceration in stressed rats, Pharmacology, 32, 326, 10.1159/000138188

Levine, 1971, A method for rapid production of stress ulcer in rats, Peptic Ulcer, 92

Litchfield, 1949, A simplified method of evaluating dose-effect experiments, J. Pharm. Exper. Ther., 95, 99

Mizui, 1983, Effect of polyamines on acidified ethanol-induced gastric lesion in rats, Japan J. Pharmacol., 33, 939, 10.1016/S0021-5198(19)52438-6

Morimoto, 1991, Effect of the new anti-ulcer agent KB-5492 on experimental gastric mucosal lesions and gastric mucosal defensive factors, as compared to those of teprenone and cimetidine, Japan J. Pharmacol., 57, 495, 10.1016/S0021-5198(19)39795-1

Murakami, 1985, Pathophysiology and pathogenesis of acute gastric mucosal lesions after hypothermic restraint stress in rats, Gastroenterology, 88, 660, 10.1016/0016-5085(85)90133-7

Olfert, 1993, Guide to the Care and use of Experimental Animals. Canadian Council on Animal Care, 1

Robert, 1979, Cytoprotection by prostaglandins in rats. Prevention of gastric necroses produced by alcohol, HCl, NaOH, hypotonic and thermal injury, Gastroenterology, 77, 433, 10.1016/0016-5085(79)90002-7

Shay, 1945, A simple method forthe uniform production of gastric ulceration in the rat, Gastroenterology, 5, 43

Brito, 1997, Ethnopharmacology and sustainable development of new plant-derived drug, Ciência e Cultura, 49, 402

Brito, 1998, Antiulcerogenic activity of trans-dehydrocrotonin from Croton cajucara Benth, Planta Med., 64, 126, 10.1055/s-2006-957388

Szabo, 1987, Mechanisms of mucosal injury in the stomach and duodenum: time-sequence analysis of morphologic, functional, biochemical and histochemical studies, Scand. J. Gastroenterol., 22, 21, 10.3109/00365528709090946

Wallace, 1985, Role of prostanoids in the protective action of BW755C on the gastric mucosa, Eur. J. Pharmacol., 115, 45, 10.1016/0014-2999(85)90582-5

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Thông tin xuất bản

Journal of Pharmacy and Pharmacology

Tập 51 Số 3

341-346

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