Effects of Lactobacillus salivarius-containing tablets on caries risk factors: a randomized open-label clinical trial
Tóm tắt
To evaluate the effects of the lactic acid bacterium Lactobacillus salivarius on caries risk factors. The study was performed in 64 healthy volunteers to evaluate the effects of L. salivarius-containing tablets on caries risk factors. The participants were divided randomly into four groups, and took tablets containing L. salivarius WB21, L. salivarius TI 2711, Ovalgen® DC (antibody against glucosyltransferase from Streptococcus mutans), or xylitol. Levels of mutans streptococci and lactobacilli, amount of salivary flow, salivary pH, and salivary buffering capacity were assessed before and after taking the tablets. Subsequently, a short-term administration trial using L. salivarius WB21-containing tablets was performed in eight healthy volunteers. The participants took L. salivarius WB21-containing tablets (2.0 × 109 colony forming units/day) for 2 weeks, and the numbers of mutans streptococci in saliva were counted. The levels of mutans streptococci seemed to decrease in the L. salivarius WB21, TI 2711, and Ovalgen® DC groups compared to the xylitol group, with no significant differences between the groups. Lactobacilli levels significantly increased in the L. salivarius WB21 and TI 2711 groups compared to the other groups. Concerning salivary flow and salivary pH, no significant differences were observed between the groups. The salivary buffering capacity significantly increased in the L. salivarius TI 2711 group (P = 0.003) and Ovalgen® DC group (P = 0.002) compared to the xylitol group. The short-term administration trial showed that the L. salivarius WB21-containing tablets significantly decreased the number of mutans streptococci (P = 0.039).
L. salivarius-containing tablets were suggested to increase resistance to caries risk factors.
UMIN000013160
(registration date: February 14, 2014).
Tài liệu tham khảo
Gotteland M, Brunser O, Cruchet S: Systematic review: are probiotics useful in controlling gastric colonization by Helicobacter pylori?. Aliment Pharmacol Ther. 2006, 15: 1077-1086.
Mazmanian SK, Lui C, Tzianaboz AO, Kasper DL: An immunomodulatory molecule of symbiotic bacteria directs maturation of the host immune system. Cell. 2005, 122: 107-118. 10.1016/j.cell.2005.05.007.
Chen YS, Jan RL, Lin YL, Chen HH, Wang JY: Randomized placebo-controlled trial of lactobacillus on asthmatic children with allergic rhinitis. Pediatr Pulmonol. 2010, 45: 1111-1120. 10.1002/ppul.21296.
Uccello M, Malaguarnera G, Basile F, D’agata V, Malaguarnera M, Bertino G, Vacante M, Drago F, Biondi A: Potential role of probiotics on colorectal cancer prevention. BMC Surg. 2012, 12: S35-10.1186/1471-2482-12-S1-S35. doi:10.1186/1471-2482-12-S1-S35
Ahola AJ, Yli-Knuuttila H, Suomalainen T, Poussa T, Ahlström A, Meurman JH, Korpela R: Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. Arch Oral Biol. 2002, 47: 799-804. 10.1016/S0003-9969(02)00112-7.
Romani Vestman N, Hasslöf P, Keller MK, Granström E, Roos S, Twetman S, Stecksén-Blicks C: Lactobacillus reuteri influences regrowth of mutans streptococci after full-mouth disinfection: a double-blind, randomized controlled trial. Craies Res. 2013, 47: 338-345. 10.1159/000347233.
Shimauchi H, Mayanagi G, Nakaya S, Minamibuchi M, Ito Y, Yamaki K, Hirata H: Improvement of periodontal condition by probiotics with Lactobacillus salivarius WB21: a randomized, double-blind, placebo-controlled study. J Clin Periodontol. 2008, 35: 897-905. 10.1111/j.1600-051X.2008.01306.x.
Iwamoto T, Suzuki N, Tanabe T, Takeshita T, Hirofuji T: Effects of probiotic Lactobacillus salivarius WB21 on halitosis and oral health: an open-labeed pilot trial. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010, 110: 201-208. 10.1016/j.tripleo.2010.03.032.
Suzuki N, Tanabe K, Takeshita T, Yoneda M, Iwamoto T, Oshiro S, Yamashita Y, Hirofuji T: Effects of oil drops containing Lactobacillus salivarius WB21 on periodontal health and oral microbiota producing volatile sulfur compounds. J Breath Res. 2012, 6: 017106-10.1088/1752-7155/6/1/017106. doi:10.1088/1752-7155/6/1/017106
Suzuki N, Yoneda M, Tanabe K, Fujimoto A, Iha K, Seno K, Yamada K, Iwamoto T, Masuo Y, Hirofuji T: Lactobcaillus salivarius WB21-containing tablets for the treatment of oral malodor: a double blind, randomized, placebo-controlled crossover trial. Oral Surg Oral Med Oral Pathol Oral Radiol. 2014, 117: 462-470. 10.1016/j.oooo.2013.12.400.
Meurman JH, Stamatova I: Probiotics: contributions to oral health. Oral Dis. 2007, 13: 443-451. 10.1111/j.1601-0825.2007.01386.x.
Matsuoka T, Nakanishi M, Aiba Y, Koga Y: Mechanism of Porphyromonas gingivalis killing by Lactobacillus salivarius TI2711. J Jpn Soc Periodontol (Japanese). 2004, 46: 118-126. 10.2329/perio.46.118.
Matsuoka T, Sugano N, Takigawa S, Takane M, Yoshinuma N, Ito K, Koga Y: Effect of oral Lactobacillus salivarius TI2711 (LS1) administration on periodontopathic bacteria in subgingival plaque. J Jpn Soc Periodontol (Japanese). 2006, 48: 315-324. 10.2329/perio.48.315.
Kanasi E, Johansson I, Lu SC, Kressin NR, Nunn ME, Kent R, Tanner AC: Microbial risk markers for childhood caries in pediatricians’ offices. J Dent Res. 2010, 89: 378-383. 10.1177/0022034509360010.
Byun R, Nadkarni MA, Chhour KL, Martin FE, Jacques NA, Hunter N: Quantitative analysis of diverse Lactobcaillus species present in advanced dental caries. J Clin Microbiol. 2004, 42: 3128-3136. 10.1128/JCM.42.7.3128-3136.2004.
Hahn CL, Falkler WA, Minah GE: Microbiological studies of carious dentine from human teeth with irreversible pulpitis. Arch Oral Biol. 1991, 36: 147-153. 10.1016/0003-9969(91)90077-8.
Colloca ME, Ahumada MC, López ME, Nader-Macías ME: Surface properties of lactobacilli isolated from healthy subjects. Oral Dis. 2000, 6: 227-233.
Kõll-Klais P, Mändar R, Leibur E, Marcotte H, Hammarström L, Mikelsaar M: Oral lactobacilli in chronic periodontitis and periodontal health: species composition and antimicrobial activity. Oral Microbiol Immunol. 2005, 20: 354-361. 10.1111/j.1399-302X.2005.00239.x.
Çaglar E, Cildir SK, Ergeneli S, Sandalli N, Twetman S: Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta Odontol Scand. 2006, 64: 314-318. 10.1080/00016350600801709.
Chuang LC, Huang CS, Ou-Yang LW, Lin SY: Probiotic Lactobacillus paracasei effect on cariogenic bacterial flora. Clin Oral Investig. 2011, 15: 471-476. 10.1007/s00784-010-0423-9.
Lexner MO, Blomqvist S, Dahlén G, Twetman S: Microbiological profiles in saliva and supragingival plaque from caries-active adolescents before and after a short-term daily intake of milk supplemented with probiotic bacteria – a pilot study. Oral Health Prev Dent. 2010, 8: 383-388.
Köhler B, Bratthall D: Practical method to facilitate estimation of Streptococcus mutans levels in saliva. J Clin Microbiol. 1979, 9: 584-588.
The R project homepage. [http://www.r-project.org]
Trahan L: Xylitol: A review of its action on mutans streptococci and dental plaque – its clinical significance. Int Dent J. 1995, 45: 77S-92S.
Ribelles Llop M, Guinot Jimeno F, Mayné Acién R, Bellet Dalmau LJ: Effects of xylitol chewing gum on salivary flow rate, pH, buffering capacity and presence of Streptococcus mutans in saliva. Eur J Paediatr Dent. 2010, 11: 9-14.
Krüger C, Pearson SK, Kodama Y, Vacca Smith A, Bowen WH, Hammarström L: The effects of egg-derived antibodies to glucosyltransferases on dental caries in rats. Caries Res. 2004, 38: 9-14.
Nguyen SV, Icatio FC, Nakano T, Isogai E, Hirose K, Mizugai H, Kobayashi-Sakamoto M, Isogai H, Chiba I: Anti-cell-associated glucosyltransferase immunoglobulin Y suppression of salivary mutans streptococci in healthy young adults. J Am Dent Assoc. 2011, 142: 943-949. 10.14219/jada.archive.2011.0301.
Teughels W, Van Essche M, Sliepen I, Quirynen M: Probiotics and oral healthcare. Periodontol 2000. 2008, 48: 111-147. 10.1111/j.1600-0757.2008.00254.x.
Keller MK, Hasslöf P, Stecksén-Blicks C, Twetman S: Co-aggregation and growth inhibition of probiotic lactobacilli and clinical isolates of mutans streptococci: an in vitro study. Acta Odontol Scand. 2011, 69: 263-268. 10.3109/00016357.2011.554863.
Holts C, Alexander C, Balcke C, Moré M, Auinger A, Bauer M, Junker L, Grünwald J, Lang C, Pompejus M: Lactobacillus paracasei DSMZ16671 reduces mutans streptococci: a short-term pilot study. Probiotics Antimicrob Proteins. 2013, 5: 259-263. 10.1007/s12602-013-9148-9.
Campus G, Cocco F, Carta G, Cagetti MG, Simark-Mattson C, Strohmenger L, Lingström P: Effect of a daily dose of Lactobacillus brevis CD2 lozenges in high caries risk schoolchildren. Clin Oral Invest. 2014, 18: 555-561. 10.1007/s00784-013-0980-9.
Keller MK, Twetman S: Acid production in dental plaque after exposure to probiotic bacteria. BMC Oral Health. 2012, 12: 44-10.1186/1472-6831-12-44. doi:10.1186/1472-6831-12-44
Marttinen A, Haukioja A, Karjalainen S, Nylund L, Satokari R, Öhmen C, Holgerson P, Twetman S, Söderling E: Short-term consumption of probiotic lactobacilli has no effect on acid production of supragingival plaque. Clin Oral Investig. 2012, 16: 797-803. 10.1007/s00784-011-0584-1.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6831/14/110/prepub