Effect of the Chloroform Extract of <i>Tanacetum vulgare</i> and one of its Active Principles, Parthenolide, on Experimental Gastric Ulcer in Rats

Journal of Pharmacy and Pharmacology - Tập 51 Số 2 - Trang 215-219 - 1999
Horacio Tournier1, Guillermo Schinella1, Elba M De Balsa1, H. Buschiazzo1, Salvador Máñez2, Perla M. de Buschiazzo1
1Cátedra de Farmacología, Falcultad de Ciencias Médicas, Universidad Nacional de la Plata, CIC Provincia de Buenos Aires, Argentina
2Departament de Farmacología, Facultat de Farmàcia, Universitat de València, Spain

Tóm tắt

Abstract This study examines the anti-ulcerogenic activity of a chloroform extract of Tanacetum vulgare and purified parthenolide, the major sesquiterpene lactone found in the extract. Gastric ulcers induced by oral administration of absolute ethanol to rats were reduced dose-dependently by oral pretreatment of animals with the chloroform extract (2.5–80 mg kg−1) or parthenolide (5–40 mgkg−1). When administered 30 min before challenge with the alcohol the protection ranged between 34 and 100% for the extract and 27 and 100% for parthenolide. When the products were administered orally 24h before treatment with ethanol, 40 mg kg−1 of the extract and of the lactone reduced the mean ulcer index from 4.8 ± 0.3 for control animals to 1.4 ± 0.2 and 0.5 ± 0.1, respectively. The products also prevented alcohol-induced reduction of the number of sulphydryl groups within the gastric mucosa (50.6 ± 2.3 μg (mg protein)−1 for normal animals compared with 17.7 ± 3.0 μg (mg protein)−1 for alcohol-treated animals). Administration of the extract (80 mg kg−1) or parthenolide (40 mg kg−1) 24h before ethanol treatment restored the numbers of mucosal -SH groups to values near those found for normal animals. These results suggest that the products assayed, in particular parthenolide, might find therapeutic application, although further work is required to establish their profit/risk ratio.

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Tài liệu tham khảo

Abad, 1991, Anti-inflammatory and anti-ulcerogenic activities of organic extract of Tanacetum microphyllum in rats, Phytother. Res., 5, 179, 10.1002/ptr.2650050409

Begley, 1989, Revised structures for guaianolide α-methylenebutyrolactones from feverfew, Phytochemistry, 28, 940, 10.1016/0031-9422(89)80150-5

Berry, 1984, Feverfew faces the future, Pharm. J., 232, 611

Bohlmann, 1982, Sesquiterpene lactones and other constituents from Tanacetum parthenium, Phytochemistry, 21, 2543, 10.1016/0031-9422(82)85253-9

Boyd, 1979, High concentrations of glutathione in glandular stomach: possible implications of carcinogenesis, Science, 205, 1010, 10.1126/science.572989

Evans, 1996, Trease and Evans' Pharmacognosy, 14th, 288

Giordano, 1990, The gastric cytoprotective effect of several sesquiterpene lactones, J. Nat. Prod., 53, 803, 10.1021/np50070a004

Guardia, 1994, Mucus synthesis and sulphydryl groups in cytoprotection mediated by dehydroleucodine, a sesquiterpene lactone, J. Nat. Prod., 57, 507, 10.1021/np50106a010

Hay, 1994, Toxic inhibition of smooth muscle contractility by plant-derived sesquiterpenes caused by their chemically reactive α-methylenebutyrolactone functions, Br. J. Pharmacol., 112, 9, 10.1111/j.1476-5381.1994.tb13020.x

Johnson, 1985, Efficacy of feverfew as prophylactic treatment of migraine, Br. Med. J. Clin. Res., 291, 569, 10.1136/bmj.291.6495.569

Lowry, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6

Mordujovich-Buschiazzo, 1996, Anti-inflammatory activity of Tanacetum vulgare, Fitoterapia, 67, 319

Motilva, 1992, Effects of naringenin and quercetin on experimental chronic gastric ulcer in rat. Studies on the histological findings, Phytother. Res., 6, 168, 10.1002/ptr.2650060317

Murphy, 1988, Randomised double-blind placebo-controlled trial of feverfew in migraine prevention, Lancet, 2, 189, 10.1016/S0140-6736(88)92289-1

Nano, 1980, On a chemotype of Tanacetum vulgare L., containing sesquiterpene lactones with germacrane skeleton, Fitoterapia, 51, 135

Ognyanov, 1983, Sesquiterpene lactones and flavonoids in flowers of Tanacetum vulgare., Planta Med., 48, 181

Robert, 1979, Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl and thermal injury, Gastroenterology, 77, 433, 10.1016/0016-5085(79)90002-7

Robles, 1995, Recent studies on the zoopharmacognosy, pharmacology and neuro-toxicology of sesquiterpene lactones, Planta Med., 61, 199, 10.1055/s-2006-958055

Schinella, 1998, Anti-inflammatory principles of South American Tanacetum vulgare, J. Pharm. Pharmacol., 50, 1069, 10.1111/j.2042-7158.1998.tb06924.x

Sedlak, 1968, Estimation of total, protein-bound, and nonprotein sulphydryl groups in tissue with Ellman's reagent, Anal. Biochem., 25, 192, 10.1016/0003-2697(68)90092-4

Szabo, 1981, Sulphydryl compounds may mediate gastric cytoprotection, Science, 214, 200, 10.1126/science.7280691

Vela, 1997, Inhibition of gastric acid secretion by the aqueous extract and purified extracts of Stachytarphena cayennensis, Planta Med., 63, 36, 10.1055/s-2006-957599

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Journal of Pharmacy and Pharmacology

Tập 51 Số 2

215-219

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