Effect of seed lectins from <i>Phaseolus vulgaris</i> on the development of larvae of <i>Callosobruchus maculatus</i>; mechanism of toxicity

Journal of the Science of Food and Agriculture - Tập 35 Số 4 - Trang 373-380 - 1984
Angharad M. R. Gatehouse1, Frances M. Dewey1, John A. Dove1, Katherine A. Fenton1, Árpád Pusztai2
1University of Durham, Department of Botany, Science Laboratories, South Road, Durham, DH1 3LE
2The Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB

Tóm tắt

AbstractSeeds of the kidney bean (Phaseolus vulgaris) are toxic to developing larvae of the bruchid beetle (Callosobruchus maculatus), a major storage pest of many legumes. Insect feeding trials were carried out whereby the albumin and globulin protein fractions from seeds of P. vulgaris were incorporated into artificial seeds. Both fractions were shown to be toxic and to contain haemagglutinating activity, implicating the seed lectins as being involved in seed resistance. Further feeding trials using different P. vulgaris lectin preparations confirmed the toxicity of these lectins and suggested that it was the E‐type lectin subunits (erythrocyte‐binding) which were the major antimetabolites. Indirect immunofluorescence investigations using monospecific antisera for globulin lectins showed that the lectins, when ingested by the larvae, bound to the midgut epithelial cells. It was suggested that the mechanism of lectin toxicity in this instance is analogous to that known to occur in the rat, namely that the ingested lectin causes disruption of the epithelial cells of the larval midgut leading to breakdown of the transport of nutrients into these cells, and the absorption of potentially harmful substances. This is the first time that evidence for the mechanism of lectin toxicity has been obtained in insects.

Từ khóa


Tài liệu tham khảo

Toms G. C.;Western A.InChemotaxonomy of the Leguminosae(Harborne J. B.; Boulter D.; Turner B. L. Eds) 1971 pp.367–462.

10.1007/BF01689893

Jaffe' W. G., 1960, Über Phytotoxine aus Bohnen (Phaseolus vulgaris), Arzneim. Forsch., 10, 1012

Jaffé W. G., 1968, Heat‐labile growth‐inhibiting factors in beans (Phaseolus vulgaris), J. Nutr., 94, 203, 10.1093/jn/94.2.203

10.1016/0005-2795(73)90159-1

10.1002/jsfa.2740260205

10.1111/j.1365-2672.1974.tb00425.x

Wagh P. V., 1963, Nutritive value of red kidney beans (Phaseolus vulgaris) for chicks, J. Nutr., 80, 191, 10.1093/jn/80.2.191

10.1079/PNS19790015

10.1016/0021-9975(80)90107-3

10.1007/BF01024550

10.1126/science.1265481

10.1038/256414a0

10.1146/annurev.py.16.090178.002321

Croy R. R. D.PhD thesis Aberdeen University1977.

10.1016/0005-2795(74)90250-5

10.1038/227680a0

10.1146/annurev.bi.42.070173.002545

10.1002/jsfa.2740340405

10.1002/jsfa.2740301003

Manen J. F.PhD thesis Université de Genève 1978.

Balbiani E. G., 1890, Etudes anatomique et histologiques sur le tube digestif des Cryptops, Arch. Zool. exp. gén., 8, 1

10.1016/0021-9975(82)90021-4

10.1038/303340a0

10.1016/0021-9975(80)90108-5

Thông tin
Thông tin xuất bản

Journal of the Science of Food and Agriculture

Tập 35 Số 4

373-380

Thông tin tác giả