Effect of Raw and Roasted Phoenix dactylifera L. Seed Polyphenols Extracts on Suppression of Angiogenesis in Endothelial Cells
Tóm tắt
Date seed is a by-product of Phoenix dactylifera L. fruit which is well recognized for its polyphenols content and numerous health-beneficial effects. Due to the increasing interest in natural phytochemicals with antioxidant activities, the present study aimed to extract polyphenols from both raw and roasted date seeds and investigate the anti-angiogenic effect of these two extracts (raw and roasted date seed polyphenols extracts (DSPE) at 25 and 50 µg/mL) using human microvascular endothelial cells (HMVEC). Our results showed that both raw and roasted DSPE suppressed some angiogenesis features in a dose-dependent manner including cell proliferation, migration, and capillary-like structure formation, of which raw DSPE was more potent inhibitor than roasted DSPE. Reduction in reactive oxygen species, as well as enhancement of superoxide dismutase activity occurred using both raw and roasted date seed polyphenols extracts. However, no changes were observed in advanced oxidation protein products versus control. Taken together, our data indicated that raw and roasted DSPE possess antioxidant activity, which suggested their potential use as a source of polyphenols with anti-angiogenic properties. Nevertheless, further studies are required to explore the underlying mechanisms responsible for their anti-angiogenic activities.
Tài liệu tham khảo
Bijami A, Rezanejad F, Oloumi H, Mozafari H (2020) Minerals, antioxidant compounds and phenolic profile regarding date palm (Phoenix dactylifera L.) seed development. Sci Hortic 262:109017. https://doi.org/10.1016/j.scienta.2019.109017
FAO (2019) Food and Agriculture Organization of the United Nations-FAOSTAT. http://www.fao.org/faostat/en/#data/QC.2021
Babiker EE, Atasoy G, Özcan MM, Juhaimi FA, Ghafoor K, Ahmed IAM, Almusallam IA (2020) Bioactive compounds, minerals, fatty acids, color, and sensory profile of roasted date (Phoenix dactylifera L.) seed. J Food Process Preserv 44(7):e14495. https://doi.org/10.1111/jfpp.14495
Souda B, Rami R, Jalloul B, Mohamed D (2020) Roasted date palm seeds (Phoenix dactylifera) as an alternative coffee: chemical composition and bioactive properties. Biomass Conv Bioref. https://doi.org/10.1007/s13399-020-00896-7
Taleb H, Morris RK et al (2016) Date syrup–derived polyphenols attenuate angiogenic responses and exhibits anti-inflammatory activity mediated by vascular endothelial growth factor and cyclooxygenase-2 expression in endothelial cells. Nutr Res 36(7):636–647. https://doi.org/10.1016/j.nutres.2016.02.010
Huang YJ, Nan GX (2019) Oxidative stress-induced angiogenesis. J Clin Neurosci 63:13–16. https://doi.org/10.1016/j.jocn.2019.02.019
Martin K, Appel C (2010) Polyphenols as dietary supplements: a double-edged sword. Nutr Diet Suppl 2:1–12. https://doi.org/10.2147/NDS.S6422
Maqsood S, Adiamo O, Ahmad M, Mudgil P (2019) Bioactive compounds from date fruit and seed as potential nutraceutical and functional food ingredients. Food Chem 125522. https://doi.org/10.1016/j.foodchem.2019.125522
Habib H, Platat C, AlMaqbali F, Ibrahim W (2014) Date seed (Phoenix dactylifera) extract reduces the proliferation of pancreatic cancer cells, DNA damage and superoxide-dependent iron release from ferritin in vitro. The FASEB J 28(829):820. https://doi.org/10.1096/fasebj.28.1_supplement.829.20
Thouri A, La Barbera L, Canuti L, Vegliante R, Jelled A, Flamini G, Ciriolo MR, Achour L (2019) Antiproliferative and apoptosis-inducing effect of common Tunisian date seed (var. Korkobbi and Arechti) phytochemical-rich methanolic extract. Environ Sci Pollut Res Int 26 (36):36264–36273. https://doi.org/10.1007/s11356-019-06606-9
Al-Farsi MA, Lee CY (2008) Optimization of phenolics and dietary fiber extraction from date seeds. Food Chem 108:977–985. https://doi.org/10.1016/j.foodchem.2007.12.009
Abdelaziz DHA, Ali SA (2014) The protective effect of Phoenix dactylifera L. seeds against CCl4-induced hepatotoxicity in rats. J Ethnopharmacol 155(1):736–743. https://doi.org/10.1016/j.jep.2014.06.026
Patrignani M, González-Forte LdS (2021) Characterisation of melanoidins derived from brewers' spent grain: new insights into their structure and antioxidant activity. Int J Food Sci Technol 56(1):384–391. https://doi.org/10.1111/ijfs.14653
Bouhlali EdT, Hmidani A, Bourkhis B, Khouya T, Ramchoun M, Filali-Zegzouti Y, et al (2020) Phenolic profile and anti-inflammatory activity of four Moroccan date (Phoenix dactylifera L.) seed varieties. Heliyon 6 (2):e03436. https://doi.org/10.1016/j.heliyon.2020.e03436
Lemos MRB, Siqueira EMdA, Arruda SF, Zambiazi RC (2012) The effect of roasting on the phenolic compounds and antioxidant potential of baru nuts [Dipteryx alata Vog.]. Food Res Int 48 (2):592–597. https://doi.org/10.1016/j.foodres.2012.05.027
Galati G, Lin A, Sultan AM, O’Brien PJ (2006) Cellular and in vivo hepatotoxicity caused by green tea phenolic acids and catechins. Free Radic Biol Med 40(4):570–580. https://doi.org/10.1016/j.freeradbiomed.2005.09.014
Yang QQ, Farha AK, Cheng L, Kim G, Zhang T, Corke H (2020) Phenolic content and in vitro antioxidant activity in common beans (Phaseolus vulgaris L.) are not directly related to anti-proliferative activity. Food Biosci 36:100662. https://doi.org/10.1016/j.fbio.2020.100662
Kyselova Z (2011) Toxicological aspects of the use of phenolic compounds in disease prevention. Interdiscip Toxicol 4(4):173–183. https://doi.org/10.2478/v10102-011-0027-5
Khan F, Ahmed F, Pushparaj P, Abuzenadah A, Kumosani T, Barbour E, et al (2016) Ajwa date (Phoenix dactylifera L.) extract inhibits human breast adenocarcinoma (MCF7) cells in vitro by inducing apoptosis and cell cycle arrest. PLoS One 11:e0158963. https://doi.org/10.1371/journal.pone.0158963.
Lin S, Hu J, Zhou X, Cheung PCK (2017) Inhibition of vascular endothelial growth factor-induced angiogenesis by chlorogenic acid via targeting the vascular endothelial growth factor receptor 2-mediated signaling pathway. J Funct Foods 32:285–295. https://doi.org/10.1016/j.jff.2017.03.009
Chojnacka K, Lewandowska U (2020) The antiangiogenic activity of polyphenol-rich extracts and its implication on cancer chemoprevention. Food Rev Int 36(1):77–103. https://doi.org/10.1080/87559129.2019.1630634
Lamalice L, Le Boeuf F, Huot J (2007) Endothelial cell migration during angiogenesis. Circ Res 100(6):782–794. https://doi.org/10.1161/01.RES.0000259593.07661.1e
Kim YW, Byzova TV (2014) Oxidative stress in angiogenesis and vascular disease. Blood 123(5):625–631. https://doi.org/10.1182/blood-2013-09-512749
Hussain T, Tan B, Yin Y, Blachier F, Tossou MC, Rahu N (2016) Oxidative stress and inflammation: what polyphenols can do for us? Oxid Med Cell Longev 2016:7432797. https://doi.org/10.1155/2016/7432797
Younus H (2018) Therapeutic potentials of superoxide dismutase. Int J Health Sci 12(3):88–93
Saryono S, Rahmawati E, Proverawati A, Hisni D (2017) Effect of antioxidant status and oxidative stress products in pre-menopausal women after treatment with date seed powder (Phoenix dactylifera L.): a study on women in Indonesia. Pak J Nutr 16(6):477–81
Asakura H, Kitahora T (2018) Chapter 23 - Antioxidants and polyphenols in inflammatory bowel disease: ulcerative colitis and crohn disease. In: Watson RR, Preedy VR, Zibadi S (eds) Polyphenols: Prevention and treatment of human disease (2nd edn). Academic, Cambridge, 279–292. https://doi.org/10.1016/B978-0-12-813008-7.00023-0
Gryszczyńska B, Formanowicz D, Budzyń M, Wanic-Kossowska M, Pawliczak E, Formanowicz P et al (2017) Advanced oxidation protein products and carbonylated proteins as biomarkers of oxidative stress in selected atherosclerosis-mediated diseases. Biomed Res Int 2017:4975264. https://doi.org/10.1155/2017/4975264
Nemzer BV, Rodriguez LC, Hammond L, DiSilvestro R, Hunter JM, Pietrzkowski Z (2011) Acute reduction of serum 8-iso-PGF2-alpha and advanced oxidation protein products in vivo by a polyphenol-rich beverage; a pilot clinical study with phytochemical and in vitro antioxidant characterization. Nutr J 10(1):67. https://doi.org/10.1186/1475-2891-10-67
Krzystek-Korpacka M, Neubauer K, Berdowska I, Boehm D, Zielinski B, Petryszyn P et al (2008) Enhanced formation of advanced oxidation protein products in IBD. Inflamm Bowel Dis 14(6):794–802. https://doi.org/10.1002/ibd.20383