Jae‐Woon Jung1,2, Su-Mi Woo1,2, Bin-Na Lee3,2, Jeong‐Tae Koh2, Jacques E. Nör4, Yun‐Chan Hwang3,2
1Department of Physiology School of Dentistry Dental Science Research Institute Chonnam National University Gwangju Korea
2Research Center for Biomineralization Disorders, Chonnam National University, Gwangju, Korea
3Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
4Angiogenesis Research Laboratory Department of Cariology Restorative Sciences Endodontics School of Dentistry University of Michigan Ann Arbor MI USA
Tóm tắt
AbstractAimTo compare the mineralization inductive capacity of Biodentine and Bioaggregate with Mineral trioxide aggregate (MTA) and to investigate possible signaling pathways of mineralization in human dental pulp cells (HDPCs).MethodologyViability of HDPCs in response to Biodentine, Bioaggregate, and MTA was measured using 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide. To investigate their potential to induce odontoblast differentiation, expression of dentine sialophosphoprotein (DSPP) and dentine matrix protein1 (DMP1) mRNA level was evaluated by RT‐PCR. For the mineralized nodule assay, Alizarin red staining was performed. To determine the role of MAPK signaling in the odontoblastic differentiation of HDPCs, activated MAPKs were investigated by Western blot and the effect of MAPK inhibitor was examined by Alizarin red S staining. The results were statistically analysed using one‐way anova and the Bonferroni test.ResultsThe effects of MTA, Biodentine, and Bioaggregate on cell viability were similar. Biodentine and Bioaggregate enhanced DSPP and DMP1 mRNA expression compared to the control group, but to the same extent as MTA (P < 0.05). MTA, Biodentine, and Bioaggregate increased the area of calcified nodules compared to the control (P < 0.01). MTA, Biodentine, and Bioaggregate increased phosphorylation of extracellular signal‐regulated kinase (ERK), p38, and c‐Jun N‐terminal kinase (JNK). MAPK inhibitors attenuated mineralized nodule formation, which was increased by MTA, Biodentine, and Bioaggregate, respectively (P < 0.01).ConclusionBiodentine and Bioaggregate stimulated odontoblastic differentiation and mineralization nodule formation by activating the MAPK pathway as did MTA. This suggests that the new materials could be useful for regenerative endodontic procedures.
