Ectopic Expression of Glycine max GmNAC109 Enhances Drought Tolerance and ABA Sensitivity in Arabidopsis

Biomolecules - Tập 9 Số 11 - Trang 714
Nguyen Cao Nguyen1, Xuan Lan Thi Hoang1, Quang Nguyen1, Ngo Xuan Binh2, Yasuko Watanabe3, Nguyễn Phương Thảo1, Lam‐Son Phan Tran4,5
1School of Biotechnology, International University—Vietnam National University HCMC, Ho Chi Minh 700000, Vietnam
2Faculty of Biotechnology and Food Technology, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen 250000, Vietnam
3Stress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan
4Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang, Vietnam
5Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang, Vietnam; Stress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan

Tóm tắt

The NAC (NAM, ATAF1/2, CUC2) transcription factors are widely known for their various functions in plant development and stress tolerance. Previous studies have demonstrated that genetic engineering can be applied to enhance drought tolerance via overexpression/ectopic expression of NAC genes. In the present study, the dehydration- and drought-inducible GmNAC109 from Glycine max was ectopically expressed in Arabidopsis (GmNAC109-EX) plants to study its biological functions in mediating plant adaptation to water deficit conditions. Results revealed an improved drought tolerance in the transgenic plants, which displayed greater recovery rates by 20% to 54% than did the wild-type plants. In support of this finding, GmNAC109-EX plants exhibited lower water loss rates and decreased endogenous hydrogen peroxide production in leaf tissues under drought, as well as higher sensitivity to exogenous abscisic acid (ABA) treatment at germination and early seedling development stages. In addition, analyses of antioxidant enzymes indicated that GmNAC109-EX plants possessed stronger activities of superoxide dismutase and catalase under drought stress. These results together demonstrated that GmNAC109 acts as a positive transcriptional regulator in the ABA-signaling pathway, enabling plants to cope with adverse water deficit conditions.

Từ khóa


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