Drp1 phân bố rộng rãi nhưng không đồng đều trong hệ thần kinh trung ương của chuột

Molecular Brain - Tập 13 - Trang 1-22 - 2020
Ting-Ting Luo1,2, Chun-Qiu Dai1,3, Jia-Qi Wang1, Zheng-Mei Wang1,4, Yi Yang1,4, Kun-Long Zhang1,5, Fei-Fei Wu1, Yan-Ling Yang6, Ya-Yun Wang1
1National Demonstration Center for Experimental Preclinical Medicine Education, Air Force Medical University (The Fourth Military Medical University), Xi’an, China
2Mental Health Center, West China Hospital of Sichuan University, Chengdu, China
3Third Medical District, Lintong Rehabilitation and Convalescent Centre, Xi’an, China
4Medical College of Yan’an University, Yan’an, China
5Department of Rehabilitation Physiotherapy, Xi-Jing Hospital, Air Force Medical University (The Fourth Military Medical University), Xi’an, China
6Department of Hepatobiliary Surgery, Xi-Jing Hospital, Air Force Medical University (The Fourth Military Medical University), Xi’an, China

Tóm tắt

Drp1 được biểu hiện rộng rãi trong hệ thần kinh trung ương của chuột và đóng vai trò trong quá trình phân chia ty thể. Nhiều bệnh lý có liên quan đến Drp1 và ty thể. Tuy nhiên, do phân bố chính xác của Drp1 chưa được quan sát cụ thể, nên khó khăn trong việc xác định tác động của các phân tử chống Drp1 lên cơ thể con người. Làm rõ phân bố cụ thể của Drp1 có thể là cách tiếp cận tốt để điều trị có mục tiêu hoặc dự đoán bệnh. Chúng tôi đã hình dung phân bố của Drp1 ở những vùng khác nhau của não và giải thích mối quan hệ giữa Drp1 và ty thể. Những con chuột GAD67-GFP knock-in đã được sử dụng để phát hiện các mẫu biểu hiện của Drp1 trong các nơron GABAergic. Chúng tôi cũng đã phân tích thêm biểu hiện của Drp1 trong mô glioma ác tính ở người. Drp1 được phân bố rộng rãi nhưng không đồng đều trong hệ thần kinh trung ương. Quan sát thêm cho thấy Drp1 được biểu hiện cao và không đồng đều trong các nơron ức chế. Dưới kính hiển vi điện tử truyền qua, phân bố của Drp1 cao hơn ở các nhánh rất nhiều so với các khu vực khác trong nơron, và chỉ một lượng nhỏ Drp1 được định vị trong ty thể. Trong glioma ác tính ở người, cường độ huỳnh quang của Drp1 tăng từ loại I-III, trong khi loại IV cho thấy xu hướng giảm. Trong nghiên cứu này, chúng tôi đã quan sát thấy sự phân bố rộng rãi không đồng đều của Drp1 trong hệ thần kinh trung ương, có thể liên quan đến sự xuất hiện và phát triển của bệnh lý thần kinh. Chúng tôi hy vọng rằng mối quan hệ giữa Drp1 và ty thể có thể sẽ hướng dẫn các liệu pháp điều trị trong lâm sàng.

Từ khóa

#Drp1 #hệ thần kinh trung ương #nơron GABAergic #glioma ác tính #ty thể

Tài liệu tham khảo

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Molecular Brain

Tập 13

1-22

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