Drought acclimation confers oxidative stress tolerance by inducing co‐ordinated antioxidant defense at cellular and subcellular level in leaves of wheat seedlings

Physiologia Plantarum - Tập 127 Số 3 - Trang 494-506 - 2006
Devarshi Selote1, Renu Khanna‐Chopra1
1Stress Physiology and Biochemistry Laboratory, Water Technology Centre, Indian Agricultural Research Institute, New Delhi 110012, India

Tóm tắt

Wheat (Triticum aestivum L.) seedlings of a drought‐resistant cv. C306 were subjected to severe water deficit directly or through stress cycles of increasing intensity with intermittent recovery periods (drought acclimation). The antioxidant defense in terms of redox metabolites and enzymes in leaf cells, chloroplasts, and mitochondria was examined in relation to ROS‐induced membrane damage. Drought‐acclimated seedlings modulated growth by maintaining favorable turgor potential and RWC and were able to limit H2O2 accumulation and membrane damage as compared with non‐acclimated plants during severe water stress conditions. This was due to systematic upregulation of H2O2‐metabolizing enzymes especially ascorbate peroxidase (APX, EC 1.11.1.11) and by maintaining ascorbate–glutathione redox pool in acclimated plants. By contrast, failure in the induction of APX and ascorbate–glutathione cycle enzymes makes the chloroplast susceptible to oxidative stress in non‐acclimated plants. Non‐acclimated plants protected the leaf mitochondria from oxidative stress by upregulating superoxide dismutase (SOD, EC 1.15.1.1), APX, and glutathione reductase (GR, EC 1.6.4.2) activities. Rewatering led to rapid enhancement in all the antioxidant defense components in non‐acclimated plants, which suggested that the excess levels of H2O2 during severe water stress conditions might have inhibited or downregulated the antioxidant enzymes. Hence, drought acclimation conferred enhanced oxidative stress tolerance by well‐co‐ordinated induction of antioxidant defense both at the chloroplast and at the mitochondrial level.

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Physiologia Plantarum

Tập 127 Số 3

494-506

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