Dopamine and sensory tissue development in Drosophila melanogaster

Wiley - Tập 47 Số 4 - Trang 280-294 - 2001
Wendi S. Neckameyer1, Janis M. O’Donnell2, Zhinong Huang2, W. Stark3
1Department of Pharmacological and Physiological Science, St. Louis University School of Medicine, St. Louis, Missouri 63108, USA.
2Department of Biological Sciences, University of Alabama at Tuscaloosa, Tuscaloosa, Alabama 35487
3Department of Biology, St. Louis University, St. Louis, Missouri 63108

Tóm tắt

Abstract

Dopamine is an important signaling molecule in the nervous system; it also plays a vital role in the development of diverse non‐neuronal tissues in the fruit fly Drosophila melanogaster. The current study demonstrates that males depleted of dopamine as third instar larvae (via inhibition of the biosynthetic enzyme tyrosine hydroxylase) demonstrated abnormalities in courtship behavior as adults. These defects were suggestive of abnormalities in sensory perception and/or processing. Electroretinograms (ERGs) of eyes from adults depleted of dopamine for 1 day as third instar larvae revealed diminished or absent on‐ and off‐transients. These sensory defects were rescued by the addition of L‐DOPA in conjunction with tyrosine hydroxylase inhibition during the larval stage. Depletion of dopamine in the first or second larval instar was lethal, but this was not due to a general inhibition of proliferative cells. To establish that dopamine was synthesized in tissues destined to become part of the adult sensory apparatus, transgenic lines were generated containing 1 or 4 kb of 5′ upstream sequences from the Drosophila tyrosine hydroxylase gene (DTH) fused to the E. coli β‐galactosidase reporter. The DTH promoters directed expression of the reporter gene in discrete and consistent patterns within the imaginal discs, in addition to the expected expression in gonadal, brain, and cuticular tissues. The β‐galactosidase expression colocalized with tyrosine hydroxylase protein. These results are consistent with a developmental requirement for dopamine in the normal physiology of adult sensory tissues. © 2001 John Wiley & Sons, Inc. J Neurobiol 47: 280–294, 2001

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Tài liệu tham khảo

10.1523/JNEUROSCI.12-11-04460.1992

10.1002/cne.902680309

10.1111/j.1471-4159.1989.tb08567.x

10.1017/S0952523800011263

10.1016/0925-4773(91)90030-A

Cohen S, 1993, 747

10.1111/j.1558-5646.1969.tb03540.x

10.1002/j.1460-2075.1990.tb07593.x

10.1023/A:1025962431132

10.1523/JNEUROSCI.16-12-03925.1996

10.1007/BF01053355

10.1007/BF01071319

10.1016/S0092-8674(00)81085-5

10.1101/gad.9.15.1869

10.1038/scientificamerican0495-72

10.1016/S0896-6273(00)80139-7

10.1016/0076-6879(87)55014-5

10.1038/222354a0

10.1016/S0378-1119(98)00440-5

10.1007/BF00848157

10.1074/jbc.270.45.27235

Koch P, 1998, Regulation of dopa decarboxylase expression during colour pattern formation in wild‐type and melanic tiger swallowtail butterflies, Devt, 125, 2302

10.1038/239281a0

10.3109/01677060009083474

10.1016/S0042-6989(96)00253-2

10.1073/pnas.95.18.10990

10.1073/pnas.93.19.10377

10.1016/0169-328X(94)90011-6

10.1046/j.1471-4159.1996.66010020.x

10.1006/dbio.1996.0128

10.3109/01677069809167259

10.1101/lm.5.1.157

10.1016/0896-6273(89)90183-9

Neckameyer W, 1992, A single locus encodes both phenylalanine hydroxylase and tryptophan hydroxylase activities in Drosophila, J Biol Chem, 267, 4199, 10.1016/S0021-9258(19)50648-2

10.3109/01677069309083448

10.1016/S0197-4580(99)00109-8

10.1016/B978-0-409-90042-2.50028-3

10.1002/neu.480230803

10.1093/genetics/118.3.461

10.1016/0012-1606(85)90324-0

10.1073/pnas.74.12.5463

10.1016/S0022-2836(75)80083-0

10.3109/02713688509003351

10.1016/0042-6989(72)90049-1

10.1016/0378-1119(88)90177-1

10.1016/S0960-9822(00)80083-4

10.1093/emboj/17.8.2285

10.1002/(SICI)1096-9861(19990215)404:3<285::AID-CNE1>3.0.CO;2-S

10.1093/nar/21.7.1682

10.1016/S0065-2660(08)60008-5

10.1016/0092-8674(94)90399-9

10.1073/pnas.92.12.5525

10.1016/0092-8674(95)90145-0