Diversity of Free-Living and Attached Bacteria in Offshore Western Mediterranean Waters as Depicted by Analysis of Genes Encoding 16S rRNA

Applied and Environmental Microbiology - Tập 65 Số 2 - Trang 514-522 - 1999
Silvia G. Acinas1, Josefa Antón2, Francisco Rodríguez‐Valera1
1Unidad de Microbiologı́a, Centro de Biologı́a Molecular y Celular, Universidad Miguel Hernández, Campus de San Juan, 03550 San Juan, Alicante,1 and
2División de Microbiologı́a, Departamento de Biotecnologı́a, Universidad de Alicante, 03080 Alicante,2 Spain

Tóm tắt

ABSTRACT In a previous study (S. G. Acinas, F. Rodrı́guez-Valera, and C. Pedrós-Alió, FEMS Microbiol. Ecol. 24:27–40, 1997), community fingerprinting by 16S rDNA restriction analysis applied to Mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 μm. Here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16S rDNA obtained from the same samples, and we have also studied the samples by scanning electron microscopy to detect morphology patterns. As expected, the sequences retrieved from the assemblages were very different. The subsample of attached bacteria contained very little diversity, with close relatives of a well-known species of marine bacteria, Alteromonas macleodii , representing the vast majority of the clones at every depth. On the other hand, the free-living assemblage was highly diverse and varied with depth. At 400 m, close relatives of cultivated γ Proteobacteria predominated, but as shown by other authors, near the surface most clones were related to phylotypes described only by sequence, in which the α Proteobacteria of the SAR11 cluster predominated. The new technique of rDNA internal spacer analysis has been utilized, confirming these results. Clones representative of the A. macleodii cluster have been completely sequenced, producing a picture that fits well with the idea that they could represent a genus with at least two species and with a characteristic depth distribution.

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Tài liệu tham khảo

10.1111/j.1574-6941.1997.tb00420.x

10.1128/aem.51.3.614-621.1986

10.1128/aem.62.6.2138-2144.1996

10.4319/lo.1986.31.1.0068

10.1128/mr.59.1.143-169.1995

10.1128/jb.110.1.402-429.1972

10.1128/aem.61.3.944-952.1995

10.1128/aem.63.7.2647-2653.1997

10.1139/f82-223

10.1126/science.218.4574.795

10.1139/f82-108

10.1146/annurev.mi.36.100182.000331

10.4319/lo.1993.38.5.0924

10.3354/meps042289

10.3354/meps092289

Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evaluation 39 1985 783 791

10.1128/aem.63.1.63-70.1997

10.1128/aem.54.6.1426-1429.1988

Gauthier G. Gauthier M. Christen R. Phylogenetic analysis of the genera Alteromonas,Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int. J. Syst. Bacteriol. 45 1995 755 761

10.5670/oceanog.1993.04

10.1073/pnas.93.15.7979

10.1128/aem.62.4.1171-1177.1996

10.1099/13500872-142-1-3

Herbland A. Le Borgne R. Le Bouteiller A. Voituriez B. Structure hydrologique et production primaire dans l’Atlantique tropical oriental. Oceanogr. Trop. 17 1983 15 25

10.1128/aem.56.2.483-487.1990

Jukes T. H. Cantor C. R. Evolution of protein molecules Mammalian protein metabolism. Munro H. N. 1969 21 132 Academic Press New York N.Y

10.1093/plankt/9.3.433

10.4319/lo.1991.36.7.1277

10.1038/333067a0

10.4319/lo.1987.32.5.1034

10.1093/nar/22.17.3485

10.1016/0168-6496(95)00029-A

Morita R. Y. Bacteria in oligotrophic environments. 1997 Chapman & Hall New York N.Y

10.4319/lo.1995.40.1.0148

Muyzer G. Personal communication.

10.1128/AEM.59.3.695-700.1993

10.1128/aem.63.9.3359-3366.1997

10.4319/lo.1997.42.5.0811

10.1111/j.1574-6968.1993.tb05806.x

Saitou N. Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 1987 406 425

10.1128/aem.63.3.983-989.1997

van der Maarel. Personal communication.

10.1128/aem.62.3.766-771.1996

10.1128/aem.63.4.1441-1448.1997

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Applied and Environmental Microbiology

Tập 65 Số 2

514-522

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