Distribution of hepatitis C virus RNA in the liver and its relation to histopathological changes

Wiley - Tập 16 Số 1 - Trang 55-60 - 1996
S. Kojima1, Yukië Tanaka, Nobuyuki Enomoto, Fumiaki Marumo, Chifumi Sato
1Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

Tóm tắt

Abstract: To investigate a cellular mode of HCV‐infection in the liver and its pathological implications in relation to histopathological changes or clinical data, we studied the distribution of HCV‐RNA in the livers of 21 patients with HCV‐related chronic liver disease (chronic active hepatitis, 14 cases; cirrhosis, 7 cases) using the in situ hybridization technique. In situ hybridization was performed on 4% paraformaldehyde‐fixed frozen sections with digoxigenin‐labeled DNA probe deduced from the core region of HC–J4. In situ hybridization showed positive signals in the liver specimens of 20/21 cases. The signals were localized in the cytoplasm of hepatocytes. The distribution pattern of positive cells was individually different, whereas the pattern was identical in the right and left lobes. There were no correlations of the HCV‐positive cell number with serum aminotransferase levels at biopsy or with genotypes of HCV. The positive hepatocytes were occasionally associated with infiltrating mononuclear cells, and they were sparsely distributed in the area of piecemeal necrosis. These findings suggest that factors such as host immunoreaction to the virus may be more important than its direct cytopathy in the pathogenesis of chronic hepatitis C virus infection.

Từ khóa


Tài liệu tham khảo

10.1002/hep.1840020509

10.1002/hep.1840150402

10.1002/hep.1840170304

10.1111/j.1600-0676.1993.tb00631.x

Chomozynski P., 1987, Single‐step method of RNA isolation by acid guanidinium thiocyanate‐phenol‐chloroform extraction, Anal Biochem, 162, 156

10.1016/0140-6736(91)92775-W

Okamoto H., 1990, Detection of hepatitis C virus RNA by a two‐stage polymerase chain reaction with two pairs of primers deduced from the 5′‐noncoding region, Jpn J Exp Med, 60, 215

10.1099/0022-1317-73-3-673

Okamoto H., 1990, The 5′‐terminal sequence of the hepatitis C virus genome, Jpn J Exp Med, 60, 167

Nakopoulou L., 1992, Expression of HBsAg and HBcAg in liver tissue: correlation with disease activity, Histol Histopathol, 7, 493

10.1007/BF02090190

10.1093/nar/13.5.1777

10.1177/42.2.8288868

10.1016/S0168-8278(05)80119-9

10.1007/BF02989207

10.1172/JCI116449

10.1016/0016-5085(92)90856-T

10.1073/pnas.89.6.2247

10.1016/S0168-8278(05)80015-7

10.1016/0168-8278(92)90073-X

10.1016/S0168-8278(05)80674-9

10.1016/S0168-8278(05)80431-3

10.1002/hep.1840150305

Shindo M., 1992, A capture assay for HCV RNA using a human monospecific antibody to core antigen of hepatitis C virus (HCV) [Abstract], Hepatology, 16, 70A

10.1007/BF01310568

10.1016/S0140-6736(94)91509-1

10.1128/JVI.67.7.3923-3930.1993

10.1099/0022-1317-75-6-1361

Thông tin
Thông tin xuất bản

Wiley

Tập 16 Số 1

55-60

Thông tin tác giả