Distribution of diacylglycerols among plasma lipoproteins in control subjects and in patients with non‐insulin‐dependent diabetes

European Journal of Clinical Investigation - Tập 29 Số 2 - Trang 139-144 - 1999
Florent Lalanne1, V. Pruneta1, Sophie Bernard2, Gabriel Ponsin1
1UNIVERSITE CLAUDE BERNARD LYON
2Hôpital de l'Antiquaille, Lyon, France

Tóm tắt

BackgroundDiacylglycerols (DAGs), which are well‐known components of insect lipophorins, have been recently recognized as a major glyceride of human high‐density lipoprotein (HDL). Moreover, DAGs are good substrates for hepatic lipase and for the phospholipid transfer protein (PLTP). The present work was undertaken to determine the lipoprotein concentrations of DAGs, in control subjects, in non‐insulin‐dependent diabetic (NIDD) patients and in patients with severe hypertriglyceridaemia.Materials and methodsLipoproteins were isolated from 11 control subjects, 17 diabetic patients and three hypertriglyceridaemic patients, using a combination of ultracentrifugation and precipitation. After lipid extraction, DAGs were separated by thin‐layer chromatography and quantified by a glyceride assay.ResultsDAGs were detectable in all lipoprotein fractions of the three groups of subjects. Total DAGs were correlated with total triglycerides (TGs) and even more strikingly with very low‐density lipoprotein triglycerides. Although the majority of DAG was recovered in apo B‐containing lipoproteins, the proportion of DAG with respect to TG was most elevated in HDL.ConclusionThese findings indicate that DAGs are probably formed from TG during lipolysis and that they can be transported to HDL through the action of PLTP. This raises the question whether DAG might act as an inhibitor of phospholipid transfer by competition for binding to PLTP.

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European Journal of Clinical Investigation

Tập 29 Số 2

139-144

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