Distribution of Genes Encoding Resistance to Macrolides, Lincosamides, and Streptogramins among Staphylococci

Antimicrobial Agents and Chemotherapy - Tập 43 Số 5 - Trang 1062-1066 - 1999
Gérard Lina1, Alain Quaglia1, Marie-Élisabeth Reverdy1, Roland Leclercq2, François Vandenesch1, Jérôme Etienne1
1Faculté de Médecine, Laboratoire de Bactériologie, Centre National de Référence des Toxémies à Staphylocoques, EA 1655, 69372 Lyon Cedex 08,1 and
2Service de Microbiologie, CHU Côte de Nacre, 14033 Caen,2 France

Tóm tắt

ABSTRACT

The relative frequency of 10 determinants of resistance to macrolides, lincosamides, and streptogramins was investigated by PCR in a series of 294 macrolide-, lincosamide-, and/or streptogramin-resistant clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci isolated in 1995 from 32 French hospitals. Resistance was mainly due to the presence of ermA or ermC genes, which were detected in 259 strains (88%), in particular those resistant to methicillin (78% of the strains). Macrolide resistance due to msrA was more prevalent in coagulase-negative staphylococci (14.6%) than in S. aureus (2.1%). Genes related to linA/linA′ and conferring resistance to lincomycin were detected in one strain of S. aureus and seven strains of coagulase-negative staphylococci. Resistance to pristinamycin and quinupristin-dalfopristin was phenotypically detected in 10 strains of S. aureus and in three strains of coagulase-negative staphylococci; it was always associated with resistance to type A streptogramins encoded by vat or vatB genes and occurred in association with erm genes. The vga gene conferring decreased susceptibility to type A streptogramins was present alone in three strains of coagulase-negative staphylococci and in combination with erm genes in 10 strains of coagulase-negative staphylococci. A combination of vga-vgb-vat and ermA genes was found in a single strain of S. epidermidis .

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