Các giai đoạn khác nhau trong quá trình hình thành và giải phóng các ngưng tụ căng thẳng

eLife - Tập 5
Joshua R. Wheeler1, Tyler Matheny1, Saumya Jain1, Robert G. Abrisch1, Roy Parker1,2
1Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, United States;
2Howard Hughes Medical Institute, University of Colorado, Boulder, United States

Tóm tắt

Các ngưng tụ căng thẳng là các cấu trúc RNA-protein (RNP) không có màng bao bọc hình thành khi sự khởi đầu dịch mã bị giới hạn và chứa một cấu trúc hai pha với các cấu trúc lõi ổn định được bao quanh bởi một lớp ít tập trung hơn. Thứ tự của quá trình lắp ráp và giải phóng của hai cấu trúc này vẫn chưa được biết rõ. Phân tích theo thời gian của quá trình lắp ráp ngưng tụ cho thấy rằng việc hình thành lõi là một sự kiện sớm trong lắp ráp ngưng tụ. Quá trình giải phóng ngưng tụ căng thẳng cũng là một quá trình theo từng bước với sự phân tán lớp bao xung quanh, tiếp theo là sự làm sạch lõi. Các can thiệp làm thay đổi sự tách pha lỏng-lỏng (LLPS) do các vùng protein không có cấu trúc cố định (IDR) của các protein liên kết RNA trong ống nghiệm có tác động ngược lại đến việc lắp ráp ngưng tụ căng thẳng trong sống. Xem xét tổng thể, những quan sát này cho rằng các ngưng tụ căng thẳng được lắp ráp thông qua một quá trình đa bước, bắt đầu bằng việc lắp ráp ổn định của các mRNP không được dịch mã vào các cấu trúc lõi, điều này có thể cung cấp nồng độ địa phương cao đủ để cho phép một LLPS địa phương được điều khiển bởi các IDR trên các protein liên kết RNA.

Từ khóa


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