Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Phân tích quy trình lai giống đậu nành thực tiễn bằng cách phát triển ZDX1, một mảng chức năng thông lượng cao
Tóm tắt
Chúng tôi đã phát triển mảng chức năng ZDX1 với khả năng thông qua cao để đánh giá và lựa chọn chính xác cả cha mẹ và con cái, điều này có thể thúc đẩy nhanh chóng quá trình lai giống đậu nành. Công nghệ microarray hỗ trợ việc định gen nhanh chóng, chính xác và tiết kiệm chi phí. Ở đây, sử dụng dữ liệu tái phân tích từ 2214 mẫu đậu nành đại diện, chúng tôi đã phát triển mảng chức năng có thông lượng cao ZDX1, chứa 158.959 SNP, bao phủ 90,92% các gen đậu nành và các vị trí liên quan đến các tính trạng quan trọng. Bằng cách áp dụng mảng, tổng cộng 817 mẫu được định gen, bao gồm ba quần thể con của các dòng cha mẹ ứng viên, các dòng cha mẹ và con cái của chúng từ quá trình lai giống thực tiễn. Các SNP cố định đã được xác định trong con cái, cho thấy sự chọn lọc nhân tạo trong quá trình lai giống. Bằng cách xác định các vị trí chức năng của các tính trạng mục tiêu, con cái kháng sâu bọ kén đậu nành mới và các nguồn gốc liên quan đến sự trưởng thành mới đã được xác định thông qua các tổ hợp allele, cho thấy rằng các vị trí chức năng cung cấp một phương pháp hiệu quả để sàng lọc nhanh chóng các tính trạng hoặc nguồn gen mong muốn. Đặc biệt, chúng tôi thấy rằng chỉ số lai giống (BI) là một chỉ số tốt cho việc chọn lọc con cái. Con cái ưu việt được sinh ra từ sự kết hợp của các cha mẹ có liên quan xa, với ít nhất một cha mẹ có BI cao hơn. Hơn nữa, những tổ hợp mới dựa trên hiệu suất tốt được đề xuất cho quá trình lai giống tiếp theo sau khi loại bỏ các cha mẹ dư thừa và có liên quan gần gũi. Phân tích dự đoán tuyến tính tốt nhất trên toàn diện gen (GBLUP) là phương pháp phân tích tốt nhất và đạt được độ chính xác cao nhất trong việc dự đoán bốn tính trạng khi so sánh SNP trong các vùng gen thay vì SNP toàn bộ hệ gen hoặc SNP liên gen. Độ chính xác dự đoán đã được cải thiện 32,1% bằng cách sử dụng con cái để mở rộng quần thể huấn luyện. Tóm lại, một xét nghiệm đa năng đã chứng minh rằng mảng chức năng ZDX1 cung cấp thông tin hiệu quả cho việc thiết kế và tối ưu hóa một quy trình lai giống để thúc đẩy nhanh chóng quá trình lai giống đậu nành.
Từ khóa
#đậu nành #mảng chức năng ZDX1 #lai giống #SNP #chỉ số lai giống #dự đoán tuyến tính tốt nhất #Tài liệu tham khảo
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