Dissecting the proteome of pea mature seeds reveals the phenotypic plasticity of seed protein composition

Proteomics - Tập 9 Số 2 - Trang 254-271 - 2009
Michaël Bourgeois1, Françoise Jacquin1, Vincent Savois1, Nicolas Sommerer2, Valérie Labas3, Céline Henry4, Judith Burstin1
1Unité Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses à Graines (UMR-LEG), Institut National de la Recherche Agronomique (INRA), Bretenières, France
2Unité de Recherche Protéomique, INRA, Montpellier, France
3Unité Mixte de Recherche Physiologie de la Reproduction et des Comportements, INRA/Centre National de la Recherche Scientifique (CNRS)/Haras Nationaux/Université de Tours, Nouzilly, France
4Unité de Biochimie Bactérienne, INRA, Domaine de Vilvert, France

Tóm tắt

AbstractPea (Pisum sativum L.) is the most cultivated European pulse crop and the pea seeds mainly serve as a protein source for monogastric animals. Because the seed protein composition impacts on seed nutritional value, we aimed at identifying the determinants of its variability. This paper presents the first pea mature seed proteome reference map, which includes 156 identified proteins (http://www.inra.fr/legumbase/peaseedmap/). This map provides a fine dissection of the pea seed storage protein composition revealing a large diversity of storage proteins resulting both from gene diversity and post‐translational processing. It gives new insights into the pea storage protein processing (especially 7S globulins) as a possible adaptation towards progressive mobilization of the proteins during germination. The nonstorage seed proteome revealed the presence of proteins involved in seed defense together with proteins preparing germination. The plasticity of the seed proteome was revealed for seeds produced in three successive years of cultivation, and 30% of the spots were affected by environmental variations. This work pinpoints seed proteins most affected by environment, highlighting new targets to stabilize storage protein composition that should be further analyzed.

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Thông tin
Thông tin xuất bản

Proteomics

Tập 9 Số 2

254-271

Thông tin tác giả