Direct Visualization of<i>Propionibacterium acnes</i>in Prostate Tissue by Multicolor Fluorescent In Situ Hybridization Assay

Journal of Clinical Microbiology - Tập 45 Số 11 - Trang 3721-3728 - 2007
Oleg A. Alexeyev1, Ingrid Marklund1, Beverley A. Shannon2, Irina Golovleva3, Jan Olsson1, Charlotte I.J. Andersson3, Iréne Eriksson4, Ronald J. Cohen5,2, Fredrik Elgh1,4
1Departments of Pathology
2Tissugen/Uropath Pty. Ltd
3Clinical Genetics
4Virology, Umeå University, Umeå, Sweden
5Faculty of Medicine, University of Western Australia, Perth, Western Australia, Australia

Tóm tắt

ABSTRACTProstate tissues from patients with prostate cancer and benign prostatic hyperplasia (BPH) frequently contain histological inflammation, and a proportion of these patients show evidence ofPropionibacterium acnesinfection in the prostate gland. We developed a multicolor fluorescent in situ hybridization (FISH) assay targetingP. acnes23S rRNA along with a 14-kb region of theP. acnesgenome. This assay was used to analyze prostate tissues from patients with prostate cancer and BPH.P. acnesinfection of the prostate gland was demonstrated in prostatic tissue in 5 of 10 randomly selected prostate cancer patients. FISH analysis and confocal laser microscopy imaging revealed intracellular localization and stromal biofilm-like aggregates as common forms ofP. acnesinfection in prostate tissues from both prostate cancer and BPH patients. A sequential analysis of prostate tissue from individual patients suggested thatP. acnescan persist for up to 6 years in the prostate gland. These results indicate thatP. acnescan establish a persistent infection in the prostate gland. Further study is needed to clarify the link between this bacterium and prostatic inflammation which may contribute to the development of BPH and prostate cancer.

Từ khóa


Tài liệu tham khảo

10.1007/s10552-006-0054-2

10.1128/mr.59.1.143-169.1995

10.1126/science.1084550

10.1016/S0022-5347(01)65066-3

10.1126/science.1100330

10.1097/01.ju.0000158161.15277.78

10.1126/science.284.5418.1318

10.1172/JCI200320365

10.1016/S0002-9440(10)65517-4

10.1128/jcm.34.8.2051-2051.1996

10.1128/jcm.16.5.844-846.1982

10.1016/S0161-6420(01)00561-9

10.1016/S0140-6736(03)12713-4

10.1002/pros.10020

Hiramatsu, J., M. Kataoka, Y. Nakata, K. Okazaki, S. Tada, M. Tanimoto, and Y. Eishi. 2003. Propionibacterium acnes DNA detected in bronchoalveolar lavage cells from patients with sarcoidosis. Sarcoidosis Vasc. Diffuse Lung Dis.20:197-203.

10.1016/S0022-5347(05)67987-6

10.1097/00001432-199906000-00006

10.1111/j.1365-2133.2005.06933.x

10.1002/pros.10317

10.1097/01.mou.0000193368.91823.1b

10.1016/S0022-5347(05)67145-5

10.1016/S0002-9440(10)63880-1

10.1016/S0022-5347(05)63958-4

10.1016/j.juro.2006.04.033

10.1097/01.ju.0000128817.44086.70

10.1002/pros.2990230204

Nickel, J. C., J. Downey, I. Young, and S. Boag. 1999. Asymptomatic inflammation and/or infection in benign prostatic hyperplasia. BJU Int.84:976-981.

10.1084/jem.20061297

10.1007/s10552-004-3486-6

Platz, E. A., and A. M. De Marzo. 2004. Epidemiology of inflammation and prostate cancer. J. Urol.171:S36-S40.

10.1093/aje/153.12.1152

10.1016/j.juro.2006.04.075

10.1111/j.1464-410X.2006.06273.x

10.1159/000282328

10.1128/JCM.43.10.5369-5371.2005

10.1111/j.1365-2605.1998.00110.x

10.1371/journal.ppat.0020025

10.1128/iai.49.1.116-121.1985

10.1002/path.1243

Thông tin
Thông tin xuất bản

Journal of Clinical Microbiology

Tập 45 Số 11

3721-3728

Thông tin tác giả