Differential Transmission of Isolates of the High Plains virus by Different Sources of Wheat Curl Mites

Plant Disease - Tập 86 Số 2 - Trang 138-142 - 2002
Dallas L. Seifers1, T. L. Harvey2, Raymond Louie3, Donald T. Gordon4, T. J. Martin5
1Professor, Kansas State University, Agricultural Research Center-Hays, Hays 67601-9228
2Professor, Department of Entomology, Kansas State University, Manhattan 66506.
3USDA-ARS
4Professor, Department of Plant Pathology, The Ohio State University, Wooster 44691
5Professor, Kansas State University, Agricultural Research Center-Hays.

Tóm tắt

High Plains virus (HPV) isolates from Colorado, Idaho, Kansas, Texas, and Utah were serologically related, had similar relative molecular masses (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) for the 32-kDa diagnostic HPV protein, and were transmissible and maintained free of Wheat streak mosaic virus (WSMV) by vascular puncture inoculation. Collections of wheat curl mites (Aceria tosichella Keifer; WCM) from Kansas, Montana, Nebraska, South Dakota, and Texas differentially transmitted these isolates. For collections from South Dakota and Texas, little or no HPV transmission occurred, whereas WCM from Nebraska and Montana transmitted all five isolates. The collection from Kansas mostly transmitted only one HPV isolate. Aviruliferous or viruliferous WSMV Nebraska WCM transmitted HPV at similar rates and aviruliferous Montana WCM transmitted HPV at lower levels than viruliferous Montana WCM.

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Tài liệu tham khảo

Ahn K.-K., 1998, J. Submicrosc. Cytol. Pathol., 30, 563

Bollig, D. M., and Edelstein, S. J. 1991. Immunoblotting. Pages 181-208 in: Protein Methods. Wiley-Liss, New York.

10.1099/0022-1317-34-3-475

del Rosario M. S. E., 1965, Phytopathology, 55, 1168

Jensen, S. G., and Hall, J. S. 1995. Molecular characterization of a viral pathogen infecting maize and wheat in the high plains. (Abstr) Phytopathology 85:1211.

10.1094/PD-80-1387

10.1038/227680a0

10.1016/0076-6879(86)18109-2

Little, T. M., and Hills, R. J. 1978. Agricultural Experimentation: Design and Analysis. John Wiley & Sons, New York.

Louie, R., and Seifers, D. L. 1996. Transmission of the High Plains pathogen by vascular puncture inoculation. (Abstr.) Phytopathology 86:Sl.

10.1094/PDIS.1998.82.3.311

10.1094/Phyto-60-1616

Nault L. R., 1970, Plant Dis. Rep., 54, 156

10.1094/PD-76-0564

10.1094/PD-79-0853

10.1094/PDIS.1999.83.10.905

10.1094/PD-80-0179

10.1094/PDIS.1997.81.10.1161

10.1094/PDIS.1998.82.8.875

10.1094/PD-79-1104

Slykhuis J. T., 1955, Phytopathology, 45, 116

Slykhuis J. T., 1956, Phytopathology, 46, 682