Different isoforms and post‐translational modifications of human salivary acidic proline‐rich proteins

Proteomics - Tập 5 Số 3 - Trang 805-815 - 2005
Rosanna Inzitari1, Tiziana Cabras2, Giuseppina Onnis2, Chiara Olmi1, Andrea Mastinu2, Maria Teresa Sanna2, Maria Guiseppina Pellegrini2, Massimo Castagnola1,3, Irene Messana2
1Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina, Università Cattolica, Rome
2Dipartimento di Scienze Applicate ai Biosistemi, Università di Cagliari, Italy
3Istituto per la Chimica del Riconoscimento Molecolare C.N.R, Rome, Italy

Tóm tắt

AbstractThe human salivary acidic proline‐rich proteins (aPRPs) complex was investigated by different chromatographic and mass spectrometric approaches and the main aPRPs, namely PRP‐1, PRP‐2 and PIF‐s (15 515 amu), Db‐s (17 632 amu) and Pa (15 462 amu) proteins, were detected. All these isoforms are phosphorylated at Ser‐8 and Ser‐22 and have a pyroglutamic moiety at the N‐terminus. Apart from Pa, all the other aPRPs undergo a proteolytic cleavage at Arg‐106 residue (Arg‐127 in Db‐s protein), that generates the small PC peptide (4371 amu) and PRP‐3, PRP‐4, PIF‐f (11 162 amu) and Db‐f (13 280 amu) proteins, all of which were detected. With regard to the Pa protein, the main form detected was the dimeric derivative (Pa 2‐mer, 30 922 amu) originated by a disulfide bond involving Cys‐103 residue. Besides these known isoforms, several previously undetected aPRP derivatives were found (in minor amounts): (i) the triphosphorylated derivatives of PRP‐1/PRP‐2/PIF‐s and Db‐s, showing the additional phosphate group at Ser‐17; (ii) the mono‐phosphorylated forms at either Ser‐22 or Ser‐8 of PRP‐1/PRP‐2/PIF‐s, PRP‐3/PRP‐4/PIF‐f, Db‐s and Db‐f; (iii) a nonphosphorylated form of PRP‐3/PRP‐4/PIF‐f; (iv) the triphosphorylated and diphosphorylated forms of Pa 2‐mer. Moreover, minor quantities of PRP‐3/PRP‐4/PIF‐f lacking the C‐terminal Arg (11 006 amu), and of Pa 2‐mer lacking the C‐terminal Gln (30 793 amu) were found. By this approach the different phenotypes of PRH1 locus in 59 different subjects were characterized.

Từ khóa


Tài liệu tham khảo

10.1042/bj1230455

10.1021/bi00799a013

10.1177/00220345870660021201

10.1016/S0021-9258(19)81061-X

10.1016/0003-9969(83)90022-5

Gibbons R. J., 1988, Infect. Immun., 56, 2990, 10.1128/iai.56.11.2990-2993.1988

Gibbons R. J., 1991, Infect. Immun., 59, 2948, 10.1128/iai.59.9.2948-2954.1991

10.1042/bj2550015

Azen E. A., 1987, Am. J. Hum. Genet., 41, 1035

10.1007/978-1-4613-0987-1_5

10.1177/00220345940730110701

10.1002/gepi.1370030302

10.1016/S1387-3806(02)00978-8

10.1016/S1044-0305(97)00284-5

10.1006/jmbi.1999.3310

10.1002/pmic.200390060

10.1021/pr049953c

10.1074/jbc.M404322200

10.1002/bmc.358

10.1074/jbc.M206333200

10.1002/pmic.200300638

Humphreys‐Beher M. G., 1984, J. Appl. Biochem., 6, 353

10.1016/j.archoralbio.2004.05.004

10.1074/jbc.272.16.10543

10.1021/bi9924213

Brunati A. M., 2000, Biochem. J., 351, 765, 10.1042/bj3510765

Thông tin
Thông tin xuất bản

Proteomics

Tập 5 Số 3

805-815

Thông tin tác giả