Different cytokine production and activation marker profiles in circulating cutaneous‐lymphocyte‐associated antigen+ T cells from patients with acute or chronic atopic dermatitis

Clinical and Experimental Allergy - Tập 34 Số 4 - Trang 559-566 - 2004
C. Antúnez1, Marı́a José Torres1, Cristobalina Mayorga1, J.A. Cornejo‐García1, Luis F. Santamaria‐Babí2, M. Blanca1
1Research Unit for Allergic Diseases, Allergy Service, Carlos Haya Hospital, Malaga, Spain.
2Almirall Prodesfarma, Barcelona, Spain

Tóm tắt

Summary

Background  Atopic dermatitis (AD) is an inflammatory skin disease whose lesions can have two stages: acute and chronic. In skin biopsies a biphasic pattern of cytokine expression has been shown, Th2 in acute lesions and Th1 in chronic AD lesions.

Objective  We investigated the expression of an activation marker and a homing receptor, as well as cytokine production, in different peripheral blood T cell subpopulations from AD patients with chronic (Group A) and acute lesions (Group B) and controls.

Methods  We evaluated 26 adult AD patients (12 Group A, 14 Group B) and 14 non‐atopic controls. IgE was measured by immunoassay. CD4, CD8, cutaneous‐lymphocyte‐associated antigen (CLA) and human leucocyte antigen (HLA)‐DR expression, and cytokine production (IL‐2, IL‐13, IFN‐γ, TNF‐α, IL‐10, IL‐4) were analysed in mononuclear cells by flow cytometry.

Results  In Group B there was a significant increase in eosinophil levels and a non‐significant increase in IgE. In Group A we found an increase in CLA+CD4+ cells (8.19±1.84) compared with controls (4.83±0.53) (P<0.05) and CD4+HLA‐DR+ cells in the CLA+ subpopulation (45.54±15.40) compared with controls (30.49±6.07) (P<0.05). In the CLA+CD4+ subpopulation, there was a significant increase in IL‐4, IL‐13 and TNF‐α production in Group B (12.46±7.7, 11.26±5.97, 43.92±15.55) compared with controls (5.34±3.50, 4.54±1.78, 19.29±9.97) with no differences in Group A.

Conclusion  Greater immunological differences were detected in peripheral blood from patients with acute compared with chronic lesions, especially in the circulating T cell‐subset with skin tropism that preferentially responded to cutaneous allergens. This is the first demonstration of phenotypic changes in circulating CLA+ T cells between AD patients with acute and chronic lesions.

Từ khóa


Tài liệu tham khảo

10.1016/S0140-6736(03)12193-9

Reitamo S., 2001, Tacrolimus, a new topical immunomodulatory therapy for atopic dermatitis, 107, 445

Picker LJ, 1990, A unique phenotype of skin associated lymphocytes in humans, preferential expression of the HECA-452 epitope by benign and malignant T-cells at cutaneous site, 136, 1053

10.1084/jem.181.5.1935

Akdis M, 1997, Skin‐homing, CLA+ memory T cells are activated in atopic dermatitis and regulate IgE by an IL‐13‐dominated cytokine pattern. IgG4 counter‐regulation by CLA− memory T cells, J Immunol, 159, 4611, 10.4049/jimmunol.159.9.4611

10.1046/j.1365-2133.2000.03665.x

10.1001/archderm.1992.01680210087014

Plaut M., 1990, Antigen‐specific lymphokine secretory patterns in atopic disease, J Immunol, 144, 4497, 10.4049/jimmunol.144.12.4497

Wierenga EA, 1990, Evidence for compartmentalization of functional subsets of CD2+ T lymphocytes in atopic patients, J Immunol, 144, 4651, 10.4049/jimmunol.144.12.4651

10.1007/BF00371611

10.1084/jem.173.3.775

10.1111/1523-1747.ep12555587

10.1016/S0140-6736(94)90879-6

10.1084/jem.186.10.1763

10.1172/JCI117408

10.1016/S0091-6749(96)70246-4

Akdis M, 1999, Skin homing (cutaneous lymphocyte‐associated antigen‐positive) CD8+ T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis, J Immunol, 163, 466, 10.4049/jimmunol.163.1.466

10.1046/j.1523-1747.1999.00720.x

10.1111/1523-1747.ep12331164

10.1016/S0167-5699(98)01285-7

Thepen T, 1996, Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ, an immunocytochemical study, 97, 828

Hanifin JM, 1980, Diagnostic features of atopic dermatitis, Acta Dermatovener Suppl (Stockh), 92, 44, 10.2340/00015555924447

Rajka G, 1989, Grading of the severity of atopic dermatitis, Acta Derm Venereol Suppl (Stockh), 144, 13

Picker LJ, 1993, Control of lymphocyte recirculation in man. II. Differential regulation of the cutaneous lymphocyte‐associated antigen, a tissue‐selective homing receptor for skin‐homing T cells, J Immunol, 150, 1122, 10.4049/jimmunol.150.3.1122

Leung DYM., 2000, Atopic dermatitis, new insights and opportunitis for therapeutic intervention, 105, 860

10.1016/0091-6749(83)90546-8

10.1084/jem.174.6.1461

Leyva L, 2000, Anticonvulsivant‐induced toxic epidermal necrolysis, monitoring the immunologic response, 105, 157

Teraki Y, 2001, Skin‐homing interleukin‐4 and ‐13‐producing cells contribute to bullous pemphigoid, remission of disease is associated with increased frequency of interleukin-10-producing cells, 117, 1097

10.1046/j.1365-2133.2003.05318.x

Dworzak MN, 1999, Skin‐associated lymphocytes in the peripheral blood of patients with atopic dermatitis, signs of subset expansion and stimulation, 103, 901

Piletta PA, 1996, Circulating skin‐homing T cells in atopic dermatitis. Selective up‐regulation of HLA‐DR, interleukin‐2R, and CD30 and decrease after combined UV‐A and UV‐B phototherapy, Arch Dermatol, 132, 1171, 10.1001/archderm.1996.03890340031006

Bohle B, 1998, Long‐lived Th2 clones specific for seasonal and perennial allergens can be detected in blood and skin by their TCR‐hypervariable regions, J Immunol, 160, 2022, 10.4049/jimmunol.160.4.2022

10.1056/NEJM200010053431407

Thông tin
Thông tin xuất bản

Clinical and Experimental Allergy

Tập 34 Số 4

559-566

Thông tin tác giả