Different cellular localization, translocation, and insulin‐induced phosphorylation of PKBα in HepG2 cells and hepatocytes

Journal of Cellular Biochemistry - Tập 86 Số 1 - Trang 118-127 - 2002
Noor Syed1, Kyla Nadine Horner1, Vikram Misra2, Ramji L. Khandelwal1
1Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada
2Department of Veterinary Microbiology, University of Saskatchewan, Veterinary Road, Saskatoon, SK Canada

Tóm tắt

AbstractProtein kinase B (PKB), a serine/threonine protein kinase, prevents apoptosis and promotes cellular transformation. PKB activity is stimulated by insulin. In this report, we examined the relative amounts of expression, location, and translocation upon insulin stimulation of PKBα in normal primary hepatocytes and carcinoma cells, HepG2 cells. Non‐phosphorylated PKBα was present in both types of unstimulated cells. The phosphorylated form of the enzyme was present in the nucleus of unstimulated HepG2 cells but not in normal hepatocytes. In the cytoplasm, PKBα was found in greater abundance in the hepatocytes as compared in HepG2 cells. Insulin induced the translocation of phosphorylated PKBα from the nucleus to the nuclear membrane in HepG2 cells. In contrast, insulin caused translocation and phosphorylation of PKBα from the cytosol to the plasma membrane in normal hepatocytes. In addition, there is a higher expression of PKBα in the HepG2 cells as compared to normal primary hepatocytes. These findings provide an important distinction between hepatocellular HepG2 cells and normal liver cells and suggest that the presence of constitutively active nuclear PKB in the transformed cells might be an important contributor in cell transformation and immortality of hepatoma cells. J. Cell. Biochem. 86: 118–127, 2002. © 2002 Wiley‐Liss, Inc.

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Thông tin xuất bản

Journal of Cellular Biochemistry

Tập 86 Số 1

118-127

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