Diethylcarbamazine Reduces Chronic Inflammation and Fibrosis in Carbon Tetrachloride- (CCl<sub><b>4</b></sub>-) Induced Liver Injury in Mice

Mediators of Inflammation - Tập 2014 - Trang 1-15 - 2014
Sura Wanessa Santos Rocha1,2, Maria Eduarda Rocha de França2, Gabriel Barros Rodrigues2, Karla Patrícia Sousa Barbosa2, Ana Karolina Santana Nunes2, André Filipe Pastor3, Anne Gabrielle Vasconcelos de Oliveira4, Wilma Helena de Oliveira2, Rayana Leal Luna2, Christina Alves Peixoto2
1Department of Entomology, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil
2Laboratory of Ultrastructure, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil
3Laboratory of Virology and Experimental Therapy, Aggeu Magalhães Research Center (CPqAM), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50740-465 Recife, PE, Brazil
4Laboratory of Microscopy and Microanalisis, Northeastern Center for Strategic Technologies (CETENE), Avenida Professor Luiz Freire, Cidade Universitária, 50740-540 Recife, PE, Brazil

Tóm tắt

This study investigated the anti-inflammatory effects of DEC on the CCl4-induced hepatotoxicity in C57BL/6 mice. Chronic inflammation was induced by i.p. administration of CCl40.5 μL/g of body weight through two injections a week for 6 weeks. DEC (50 mg/kg) was administered by gavage for 12 days before finishing the CCl4induction. Histological analyses of the DEC-treated group exhibited reduced inflammatory process and prevented liver necrosis and fibrosis. Immunohistochemical and immunofluorescence analyses of the DEC-treated group showed reduced COX-2, IL1β, MDA, TGF-β, andαSMA immunopositivity, besides exhibiting decreased IL1β, COX-2, NFκB, IFNγ, and TGFβexpressions in the western blot analysis. The DEC group enhanced significantly the IL-10 expression. The reduction of hepatic injury in the DEC-treated group was confirmed by the COX-2 and iNOS mRNA expression levels. Based on the results of the present study, DEC can be used as a potential anti-inflammatory drug for chronic hepatic inflammation.

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Mediators of Inflammation

Tập 2014

1-15

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