Dietary licorice root supplementation reduces diet‐induced weight gain, lipid deposition, and hepatic steatosis in ovariectomized mice without stimulating reproductive tissues and mammary gland

Molecular Nutrition and Food Research - Tập 60 Số 2 - Trang 369-380 - 2016
Zeynep Madak‐Erdogan1, Ping Gong2, Yiru Zhao2, Liwen Xu2, Kinga Wrobel1, James A. Hartman1, Michelle Wang1, Anthony Cam1, Urszula T. Iwaniec3, Russell T. Turner3, Nathan C. Twaddle4, Daniel R. Doerge4, Ikhlas A. Khan5,6, John A. Katzenellenbogen7, Benita S. Katzenellenbogen2, William G. Helferich1
1Botanical Research Center, Departments of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, USA
2Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA
3Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR, USA
4National Center for Toxicological Research, Jefferson, AR, USA
5Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, USA
6National Center for Natural Product Research, School of Pharmacy, University of Mississippi University, MS, USA
7Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA

Tóm tắt

ScopeWe studied the impact of dietary supplementation with licorice root components on diet‐induced obesity, fat accumulation, and hepatic steatosis in ovariectomized C57BL/6 mice as a menopause model.Materials and methodsWe evaluated the molecular and physiological effects of dietary licorice root administered to ovariectomized C57BL/6 mice as root powder (LRP), extracts (LRE), or isolated isoliquiritigenin (ILQ) on reproductive (uterus and mammary gland) and nonreproductive tissues important in regulating metabolism (liver, perigonadal, perirenal, mesenteric, and subcutaneous fat). Quantitative outcome measures including body weight, fat distribution (magnetic resonance imaging), food consumption, bone density and weight (Dual‐energy X‐ray absorptiometry), and gene expression were assessed by the degree of restoration to the preovariectomized health state. We characterized histological (H&E and oil red O staining) and molecular properties (expression of certain disease markers) of these tissues, and correlated these with metabolic phenotype as well as blood levels of bioactives.ConclusionAlthough LRE and ILQ provided some benefit, LRP was the most effective in reducing body weight gain, overall fat deposition, liver steatosis, and expression of hepatic lipid synthesis genes following ovariectomy. Our data demonstrate that licorice root provided improvement of multiple metabolic parameters under conditions of low estrogen and high‐fat diets without stimulating reproductive tissues.

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Molecular Nutrition and Food Research

Tập 60 Số 2

369-380

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