Dietary Antioxidant, Quercetin, Protects Sertoli‐Germ Cell Coculture from Atrazine‐Induced Oxidative Damage

Journal of Biochemical and Molecular Toxicology - Tập 26 Số 11 - Trang 477-485 - 2012
Sunny O. Abarikwu1, Aditya B. Pant2, Amos O. Abolaji3
1Department of Chemical Sciences, College of Natural Sciences, Redeemer’s University, Redemption City, Ogun State, Nigeria
2Indian Institute of Toxicology Research (Formerly Industrial Toxicology Research Centre); Lucknow; India
3Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, University of Ibadan, Ibadan, Oyo State, Nigeria

Tóm tắt

ABSTRACT

Quercetin (QT), a dietary‐derived flavonoid, is ubiquitous in fruits and vegetables and plays an important role in human health by virtue of its antioxidant function. The present study was designed to examine the effects of QT on oxidative damage that was induced by the herbicide, atrazine (ATZ), in mixed cultures of Sertoli‐germ cells. Results showed that treatment with QT increased cell viability and decreased catalase activity, malondialdehyde, and reactive oxygen species (ROS) levels. QT treatment also increased the mRNA expression of glutathione peroxidase (GSH‐Px), glutathione reductase (GR), glutathione‐S‐transferase, and superoxide dismutase‐1 and could not reversed to the control levels ATZ‐induced steady‐state mRNA levels of these antioxidant genes as well as the level of glutathione and activities of GSH‐Px and GR. QT has protective effect against ATZ‐induced oxidative stress through a reduction in ROS levels and lipid peroxidation. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:477‐485, 2012; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.21449

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Thông tin xuất bản

Journal of Biochemical and Molecular Toxicology

Tập 26 Số 11

477-485

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