Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans

Genes and Development - Tập 15 Số 20 - Trang 2654-2659 - 2001
René F. Ketting1, Sylvia E. J. Fischer1, Emily Bernstein2,3, Titia Sijen1, Gregory J. Hannon2, Ronald H.A. Plasterk1
1The Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, Utrecht, The Netherlands;
2Cold Spring Harbor Laboratory Cold Spring Harbor, New York, 11724 USA
3Graduate Program in Genetics State University of New York at Stony Brook Stony Brook, New York 11794 USA

Tóm tắt

Double-stranded RNAs can suppress expression of homologous genes through an evolutionarily conserved process named RNA interference (RNAi) or post-transcriptional gene silencing (PTGS). One mechanism underlying silencing is degradation of target mRNAs by an RNP complex, which contains ∼22 nt of siRNAs as guides to substrate selection. A bidentate nuclease called Dicer has been implicated as the protein responsible for siRNA production. Here we characterize theCaenorhabditis elegans ortholog of Dicer (K12H4.8;dcr-1) in vivo and in vitro. dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41).

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Genes and Development

Tập 15 Số 20

2654-2659

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