Di‐(2‐ethylhexyl) phthalate and bisphenol A exposure impairs mouse primordial follicle assembly in vitro

Environmental and Molecular Mutagenesis - Tập 55 Số 4 - Trang 343-353 - 2014
Teng Zhang1, Lan Li2,3, X. Qin1, Yang Zhou1, Xifeng Zhang4, Linqing Wang1, Massimo De Felici5, Hong Chen2, Guo‐Qing Qin6, Wei Shen1
1Laboratory of Germ Cell Biology, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China
2College of Animal Science and Technology Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling Shaanxi China
3Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University Laboratory of Germ Cell Biology Qingdao China
4College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China
5Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy
6Inno Tech Nutrition Solutions 715 Marion Street Winnipeg Manitoba Canada

Tóm tắt

Bisphenol‐A (BPA) and diethylhexyl phthalate (DEHP) are estrogenic compounds widely used in commercial plastic products. Previous studies have shown that exposure to such compounds have adverse effects on various aspects of mammalian reproduction including folliculogenesis. The objective of this study was to examine the effects of BPA and DEHP exposure on primordial follicle formation. We found that germ cell nest breakdown and primordial follicle assembly were significantly reduced when newborn mouse ovaries were exposed to 10 or 100 μM BPA and DEHP in vitro. Moreover, BPA and DEHP exposure increased the number of TUNEL positive oocytes and the mRNA level of the pro‐apoptotic gene Bax in oocytes. These effects were associated with decreased expression of oocyte specific genes such as LIM homeobox 8 (Lhx8), factor in the germline alpha (Figla), spermatogenesis and oogenesis helix‐loop‐helix (Sohlh2), and newborn ovary homeobox (Nobox). Interestingly, BPA and DEHP exposure also prevented DNA demethylation of CpG sites of the Lhx8 gene in oocytes, a process normally associated with folliculogenesis. Finally, folliculogenesis was severely impaired in BPA and DEHP exposed ovaries after transplantation into the kidney capsules of immunodeficient mice. In conclusion, BPA and DEHP exposures impair mouse primordial follicle assembly in vitro. Environ. Mol. Mutagen. 55:343–353, 2014. © 2014 Wiley Periodicals, Inc.

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Environmental and Molecular Mutagenesis

Tập 55 Số 4

343-353

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