Developmental regulation of glutamate transporters and glutamine synthetase activity in astrocyte cultures differentiatedin vitro

International Journal of Developmental Neuroscience - Tập 17 - Trang 173-184 - 1999
Danica B. Stanimirovic1, Rita Ball1, Daniel L. Small1, Arumugam Muruganandam1
1Cellular Neurobiology Group, Institute for Biological Sciences, National Research Council of Canada, Ottawa, ONT, Canada K1A 0R6

Tóm tắt

Abstract

Glutamate plays an important role in brain development, physiological function, and neurodegeneration. Astrocytes control synaptic concentration of glutamate via the high affinity glutamate transporters, GLT‐1 and GLAST, and the glutamate catabolizing enzyme, glutamine synthetase. In this study we show that astrocytes cultured from rat brain in various stages of development including embryonic (E18), postnatal (P1–P21) and mature (P50), show distinct patterns of GLT‐1 and GLAST expression, glutamine synthetase activity, and phenotypic changes induced by dibutyryl‐cyclic adenosine monophosphate. The transcripts for GLT‐1 message were detectable in embryonic astrocytes only, whereas the GLAST message was highly expressed in E18 and P1–P4 astrocyte cultures, declined in P10–P21, and was undetectable in P50 astrocytes. Uptake of3H‐glutamate correlated well with GLAST expression in astrocyte cultures of all developmental stages. Glutamine synthetase activity significantly declined from high embryonic levels in P4 astrocytes and remained low throughout postnatal maturation. Exposure of astrocyte cultures to the differentiating agent, db‐cAMP (250–500 μM; 6 days), resulted in a pronounced stellation, up‐regulation of GLT‐1 and GLAST in E18, and GLAST in P4 cultures, while it was ineffective in P10 astrocytes. By contrast, db‐cAMP induced a more pronounced stimulation of glutamine synthetase activity (up to 10‐fold above basal) in P10 than in E18 cultures (up to 2 times above basal). The differences in expression/inducibility of glutamate transporters and glutamine synthetase observed in astrocyte cultures derived from various stages of fetal and postnatal development suggest that astrocytes in vivo might also respond differently to environmental or injurious stimuli during development and maturation.


Tài liệu tham khảo

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