Development of a new initial breeding material in sunflower (Helianthus annuus L.) using direct organogenesis and somatic embryogenesis
Tóm tắt
Immature zygotic embryos from three inbred lines of sunflower (Helianthus annuus L.) were used as donor material for the induction of direct organogenesis and somatic embryogenesis. The scope of the spontaneously appearing somaclonal variation has been studied among the regenerants produced according to the method of Freyssinet & Freyssinet (1988), and was compared to the results obtained after gamma ray mutagenesis (7 and 10 Gy). Freshly excised immature zygotic embryos were used for irradiation treatment. Genetic changes occurring spontaneously during the regeneration procedure were observed for date of flowering, vegetation period, plant height, head diameter, 1000 seed weight, kernel oil content, and fatty acid composition. The mutagenic treatment had a marked stimulatory effect on the frequency of the regenerants produced and the degree of the observed changes. The 7 Gy treatment was most efficient for the majority of the characters studied. The type of changes was correlated with the genotype. The degree and type of somaclonal variation, with or without mutagenic treatment, should be sufficient for an application in the production of new breeding material.
Tài liệu tham khảo
Burchfield, H.P. & E.E.Storrs, 1962. Biochemical applications of gas chromatography. Academic Press, New York and London.
Chaleff, R.S., 1983. Isolation of agronomically useful mutants from plant cell cultures. Science 214: 676–682.
Cheng, X.Y., M.W.Gao, Z.Q.Liang & K.Z.Lin, 1990. Effect of mutagenic treatments on somaclonal variation in wheat (Triticum aestivum L.). Plant Breeding 105: 47–52.
Espinasse, A. & C.Lay, 1989. Shoot regeneration of callus derived from globular torpedo embryos from 59 sunflower genotypes. Crop Sci. 29: 201–205.
Finer, J.J., 1987. Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus anuus L.). Plant Cell Reports 6: 372–374.
Freyssinet, M. & G.Freyssinet, 1988. Fertil plant regeneration from sunflower (Helianthus annuus L.) immature embryos Plant Science 56: 177–181.
Knittel, N., A.S.Escandon & G.Hahne, 1991. Plant Regeneration at high frequency from mature sunflower cotyledons. Plant Science 73: 219–226.
Larkin, P.J. & W.R.Scowcroft, 1981. Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197–214.
Larkin, P.J., S.A.Ryan, R.L.Brettell & W.R.Scowcroft, 1984. Heritable somaclonal variation in wheat. Theor. Appl. Genet. 67: 443–455.
Larkin, P.J., 1987. Somaklonal variation: History, Method and Meaning. Iowa state Journal of research. 61: 393–434.
Murashige, T., 1974. Plant propagation through tissue culture. Ann. Rev. Plant Physiol. 25: 135–166.
Newport Instruments Ltd, 1972. Use of the Newport quantity analyzer as a replacement for solvent extraction for measuring the oil and fat content of oil seeds, chocolate, meat and other materials. Newport Pagnell, England.
Novak, F.J., S.Daskalov, H.Brunner, M.Nesticky, R.Afza, M.Dolezelova, S.Lucretti, A.Herichova & T.Hermelin, 1988. Somatic embryogenesis in maize and comparison of genetic variability induced by gamma radiation and tissue culture techniques. Plant Breeding 101: 66–79.
Paterson, K.E. & N.P.Everett, 1985. Regeneration of Helianthus annuus inbred plants from callus. Plant Sci. 42: 125–132.
Pugliesi, C., F.Cecconi, A.Mandolfo & S.Baroncelli, 1991. Plant regeneration and genetic variability from tissue culture of sunflower (Helianthus annuus L.). Plant Breeding 106: 114–121.
Roseland, C.R., A.Espinasse & T.J.Grosz, 1991. Somaclonal variants of sunflower with modified coumarin expression under stress. Euphytica 54: 183–190.
Secor, G.A. & J.F.Shepard, 1981. Variability of protoplast derived potato clones. Crop. Sci. 21: 102–105.
Skirvin, R.M., 1978. Natural and induced varition in tissue culture. Euphytica 27: 241–266.
Snedecor, G. & W.Cochran, 1957. Statistical Methods, The Iowa State College Press, Ames, Iowa.
Thomas, E.P. & D.Pratt, 1982. Isolation of paraquat tolerant mutants from tomato cell cultures. Theor. Appl. Genet. 63: 169–176.
Thomas, E.P., S.W.J.Bright, J.Franklin, V.A.Lancaster & B.J.Milvin, 1982. Variation amongst protoplast-derived potato plants (Solanum tuberosum cv. Maris Bard). Theor. Appl. Genet. 62: 65–68.
Witrzens, B., W.R.Scowcroft, R.W.Downes & P.J.Larkin, 1988. Tissue culture and plant regeneration from sunflower (Helianthus annuus L.) and interspecific hybrids (H. tuberosus × H. annuus L.). Plant cell, Tissue and Organ Culture 13: 61–76.
Wilcox Mc Cann, A., G.Cooley & J.VanDreser, 1988. A system for routine regeneration of sunflower (Helianthus annuus L.) from immature embryo-derived callus. Plant Cell, Tissue and Organ Culture 14: 104–110.