Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Phát triển Cartridge SPE thông qua để xác định nhanh chóng Fipronil và các chuyển hóa của nó trong trứng gà bằng LC-MS/MS
Tóm tắt
Trong nghiên cứu này, một cartridge làm sạch thông qua mới đã được phát triển cho việc xác định nhanh chóng fipronil và các chuyển hóa của nó trong trứng gà bằng phương pháp sắc ký lỏng ghép khối (LC-MS/MS). Các thí nghiệm loại bỏ hệ thống trên các sắc tố, lipid, protein, v.v. đã được thực hiện, và sau đó một cartridge SPE đa chức năng chứa ba chất hấp thụ (PestiCarb, Alumina-N và COOH) đã được thiết kế. Sử dụng cartridge này, một quy trình làm sạch đơn giản với hiệu quả loại bỏ lý tưởng đã được thực hiện bằng trọng lực trong dưới 1 phút. Phương pháp LC-MS/MS liên quan đã cung cấp hiệu suất hài lòng. Các chỉ số phục hồi được kiểm tra nằm trong khoảng 78.6–87.1% với độ lệch chuẩn tương đối (RSD) nhỏ hơn 8.3%. Giới hạn định lượng (LOQ) của fipronil, fipronil-desulfinyl, fipronil-sulfone và fipronil-sulfide lần lượt là 1, 0.5, 1 và 0.5 μg/kg. Phương pháp này đã được áp dụng thành công để phát hiện một số trứng gà từ các chợ và trang trại địa phương. Một mẫu dương tính với 5.3 μg/kg fipronil và 31.8 μg/kg fipronil-sulfone đã được phát hiện và nguồn gốc của nguy cơ tiềm ẩn đã được truy vết. Trong tương lai, một thiết bị cụ thể được trang bị nhiều cartridge đã phát triển trên có thể được thiết kế cho quy trình làm sạch nhanh và quy mô lớn. Điều này sẽ hữu ích để giảm bớt lao động thủ công và tiết kiệm thời gian chuẩn bị mẫu.
Từ khóa
#fipronil #trứng gà #sắc ký lỏng ghép khối #làm sạch thông qua #cartridge SPETài liệu tham khảo
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