Shuang‐Hu Cai1,2,3, Bei Wang1,2,3, K. Felix1,2,3, Jichang Jian1,2,3, Zao‐He Wu1,2,3
1Fisheries College, Guangdong Ocean University, Zhanjiang, China
2Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China
3Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China
Tóm tắt
AbstractStreptococcus iniae is a major pathogen that causes sever economic losses in tilapia aquaculture. A set of four specific primers was designed by targeting lctO gene. With Bst DNA polymerase, the target DNA can be clearly amplified for 60 min at 64 °C in a simple water bath. The sensitivity of the LAMP assay for the detection of S. iniae is about 12.4 cells per reaction in both of pure cultures and added fish tissues cultures. LAMP products could be judged with agar gel or naked eye after addition of SYBR Green I. There were no cross‐reactions with other bacterial strains indicating high specificity of the LAMP. The LAMP method was also applied to detect S. iniae ‐infected tilapia tissues effectively. The LAMP assay reported here indicates the potential usefulness of the technique as a valuable simple, rapid alternative procedure for the detection of S. iniae during streptococcicosis monitoring of cultured fish. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
