Development and differentiation of haploid Lycopersicon esculentum (tomato)
Tóm tắt
Haploid callus cultures of selected races of Lycopersicon (tomato) species can be obtained from anther culture. This is a further demonstration of a proposed general method of haploid culture developed with Arabidopsis thaliana. Differentiation of haploid callus of Lycopersicon esculentum can be controlled both in the dark and the light by hormones added to defined minimal media. Development to plantlets is achieved only in the light. Callus cells can be induced to develop into seedless pseudo-fruits. Chromosome counts on callus cells or root-tip cells establishes haploidy (n=12). Haploidy can be maintained in culture on defined minimal media for at least one year.
Tài liệu tham khảo
Blaydes, D. F.: Interaction of kinetic and various inhibitors in the growth of soy bean tissue. Physiol. Plantar. 19, 748–753 (1966).
Darlington, C. D., LaCour, L. F.: Handling of chromosomes, 2nd edn., revised. London: George Allen and Unwin 1947.
Doy, C. H., Gresshoff, P. M., Rolfe, B.: Phage-mediated transfer and subsequent expression of the galactose operon from Escherichia coli in cells of haploid tomato callus. Proc. Austr. Biochem. Soc. 5 (1972a).
Doy, C. H., Gresshoff, P. M., Rolfe, B.: Transgenosis of bacterial genes from Escherichia coli to cultured cells of haploid Lycopersicon esculentum. Proc. Int. Meeting “The biochemistry of gene expression in higher organisms”. (1972b).
Gamborg, O. L., Eveleigh, D.: Culture methods and detection of glucanases in suspension cultures of wheat and barley. Canad. J. Biochem. 46, 417–421 (1968).
Gresshoff, P. M., Doy, C. H.: Haploid Arabidopsis thaliana callus and plants from anther culture. Aust. J. biol. Sci. 25, 259–264 (1972).
Guha, S., Maheshwari, S. C.: Cell division and differentiation of embryos in pollen grains of Datura in vitro. Nature (Lond.) 212, 97–98 (1966).
Nataka, K., Tanaka, M.: Differentiation of embryoids from developing germ cells in anther culture of tobacco. Jap. J. Genet. 43, 65–71 (1968).
Sunderland, N., Wicks, F. M.: Cultivation of haploid plants from tobacco pollen. Nature (Lond.) 244, 1227–1229 (1969).
Sunderland, N., Wicks, F. M.: Embryoid formation in pollen grains of Nicotiana tabacum. J. exp. Bot. 22, 213–221 (1971).
Sunderland, N.: Anther culture: a progress report. Sci. Progr. Oxf. 59, 527–549 (1971).