Determination of protein synthesis in rainbow trout, <i>Oncorhynchus mykiss</i>, using a stable isotope

Journal of Experimental Biology - Tập 189 Số 1 - Trang 279-284 - 1994
CG Carter1, Owen Sf1,2, He Zy1, P. Watt2, Charles M. Scrimgeour2, D. F. Houlihan1, M. J. Rennie2
1University of Aberdeen 1 Department of Zoology , , Tillydrone Avenue, Aberdeen AB9 2TN, UK
2University of Dundee 2 Department of Anatomy and Physiology , , Dundee DD1 4HN, UK

Tóm tắt

ABSTRACT It has been suggested (Houlihan, 1991) that the consumption of 1 g of protein in a variety of species of fish stimulates the synthesis of, approximately, an equal amount of protein. Although synthesis of protein may account for as much as 40 % of the whole-animal oxygen consumption (Lyndon et al. 1992), only about 30 % of the synthesized proteins are retained as growth (Houlihan et al. 1988; Carter et al. 1993a,b). Thus, one focus of attention is the potential advantage gained by fish in allocating a considerable proportion of assimilated energy to protein turnover in contrast to relatively low-cost, low-turnover protein growth (Houlihan et al. 1993). Rates of protein synthesis in several species of fish have been measured using radioactively labelled amino acids, frequently given as a flooding dose (reviewed by Fauconneau, 1985; Houlihan, 1991). These measurements cannot be made for longer than a few hours because of the decline in specific radioactivity in the amino acid free pool. However, as protein synthesis rates vary during the course of a day as a result of the post-prandial stimulation, and since radiolabelled amino acid methodology is invasive, short-term and terminal, it has been difficult to be certain of the relationship between protein growth measured in the long term and protein synthesis rates measured in the short term. This paper addresses these problems by developing a method using 15N in orally administered protein to measure protein synthesis rates in fish over relatively long periods, the aim being to use procedures that are as non-invasive and repeatable as possible. The use of stable isotopes to measure protein metabolism is well established in terrestrial mammals (see Rennie et al. 1991; Wolfe, 1992), but to our knowledge the only published data for aquatic ectotherms are on the blue mussel (Mytilus edulis L.) (Hawkins, 1985). In the present study, rates of protein synthesis of individual rainbow trout [Oncorhynchus mykiss (Walbaum)] were calculated from the enrichment of excreted ammonia with 15N over the 48 h following the feeding of a single meal (dose) containing protein uniformly labelled with 15N by use of an end-point stochastic model (Waterlow et al. 1978; Wolfe, 1992). Application of this type of modelling would appear to be ideal for measuring ammonotelic fish nitrogen metabolism since, unlike the situation in mammals, the catabolic flux of amino acids through urea is very small. Further, ammonia is excreted directly into the surrounding water via the gills and is not stored for any length of time, in contrast to the situation in mammals, so the rate of tracer appearance is easily measurable.

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Tài liệu tham khảo

Carter, 1991, The bioenergetics of grass carp, Ctenopharyngodon idella (Val.): energy allocation at different planes of nutrition, J. Fish Biol, 39, 873, 10.1111/j.1095-8649.1991.tb04416.x

Carter, 1993, The relationships between protein intake and protein accretion, synthesis and retention efficiency for individual grass carp, Ctenopharyngodon idella (Val, Can. J. Zool, 71, 392, 10.1139/z93-055

Carter, 1993, Protein-nitrogen flux and protein growth efficiency of individual Atlantic salmon (Salmo salar L, Fish Physiol. Biochem, 12, 305, 10.1007/BF00004415

Fauconneau, 1985, Protein synthesis and protein deposition in fish, In Nutrition and Feeding in Fish, 17

Fauconneau, 1985, In vivo protein synthesis in different tissues and the whole body of rainbow trout (Salmo gairdneri R.). Influence of environmental temperature, Comp. Biochem. Physiol, 82A, 179, 10.1016/0300-9629(85)90723-6

Garlick, 1980, A rapid and convenient technique for measuring the rate of protein synthesis in tissues by the injection of 3H phenylalanine, Biochem. J, 217, 507

Gnaiger, 1984, Proximate biochemical composition and caloric content calculated from elemental CHN analysis: a stoichiometric concept, Oecologia (Berlin), 62, 289, 10.1007/BF00384259

Hawkins, 1985, Relationships between the synthesis and breakdown of protein, dietary absorption and turnovers of nitrogen and carbon in the blue mussel, Mytilus edulis L, Oecologia (Berlin), 66, 42, 10.1007/BF00378550

Houlihan, 1991, Protein turnover in ectotherms and its relationship to energetics, Adv. comp. env. Physiol, 7, 1, 10.1007/978-3-642-75897-3_1

Houlihan, 1988, Growth rates and protein turnover in cod, Gadus morhua, Can. J. Fish. aquat. Sci, 45, 951, 10.1139/f88-117

Houlihan, 1993, Biochemical correlates of growth rate in fish, In Fish Ecophysiology, 45, 10.1007/978-94-011-2304-4_2

Lyndon, 1992, The effect of short term fasting and a single meal on protein synthesis and oxygen consumption in cod, J. comp. Physiol. B, 162, 209, 10.1007/BF00357525

Mccarthy, 1993, Feeding behaviour and protein turnover in fish, PhD thesis, University of Aberdeen, 153

Millward, 1975, Skeletal-muscle growth and protein turnover, Biochem. J, 150, 235, 10.1042/bj1500235

Randall, 1987, Ammonia distribution in fish, Fish. Physiol. Biochem, 3, 107, 10.1007/BF02180412

Rennie, 1991, Applications of stable isotope tracers in studies of human metabolism, In New Techniques in Nutritional Research, 3

Waterlow, 1978, Protein Turnover in Mammalian Tissues and in the Whole Body

Wilkie, 1991, Nitrogenous waste excretion, acid–base regulation and ionoregulation in rainbow trout (Oncorhynchus mykiss) exposed to extremely alkaline water, Physiol. Zool, 64, 1069, 10.1086/physzool.64.4.30157957

Wolfe, 1992, Radioactive and Stable Isotope Tracers in Biomedicine: Principles and Practice of Kinetic Analysis

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Journal of Experimental Biology

Tập 189 Số 1

279-284

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